Nakachew Endalech, Melake Addisu, Alemu Marye, Eshetie Tewodros, Berhane Nega
Department of Medical Biotechnology, Institute of Biotechnology, University of Gondar, Gondar, Ethiopia.
Department of Biomedical Science, College of Health Science, Debre Tabor University, Debre Tabor, Ethiopia.
Metab Syndr Relat Disord. 2025 Feb;23(1):23-29. doi: 10.1089/met.2024.0157. Epub 2024 Dec 18.
Type 2 diabetes mellitus (T2DM) is a complex and diverse illness that is influenced by several vulnerable genes as well as environmental risk factors. The aim of this study was to ascertain the relationship between the vitamin D receptor gene polymorphisms and the risk of T2DM at the University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia. An age- and sex-matched hospital-based case-control study involved 70 patients with T2DM and 70 nondiabetic healthy controls. Demographic information was assessed in order to identify the associated risk variables. To ascertain the genotypes, DNA was taken from blood samples and used in a polymerase chain reaction and agarose gel electrophoresis analysis. The frequency of the homozygous -tt genotype [odds ratios (OR): 2.69; 95% confidence level (CL): 1.05-6.44; = 0.38], and the t allele (OR: 1.90; 95% CL: 1.16-3.12; = 0.0099) was significantly higher in patients with T2DM compared to nondiabetic controls. The results suggest that the gene polymorphism may be related to the onset of T2DM in the Ethiopian population under study.
2型糖尿病（T2DM）是一种复杂多样的疾病，受多种易感基因以及环境风险因素影响。本研究旨在确定埃塞俄比亚西北部贡德尔大学综合专科医院维生素D受体基因多态性与T2DM风险之间的关系。一项基于医院的年龄和性别匹配的病例对照研究纳入了70例T2DM患者和70例非糖尿病健康对照。评估人口统计学信息以确定相关风险变量。为确定基因型，从血样中提取DNA并用于聚合酶链反应和琼脂糖凝胶电泳分析。与非糖尿病对照相比，T2DM患者中纯合子-tt基因型的频率[比值比（OR）：2.69；95%置信水平（CL）：1.05 - 6.44；P = 0.38]以及t等位基因（OR：1.90；95% CL：1.16 - 3.12；P = 0.0099）显著更高。结果表明，在所研究的埃塞俄比亚人群中，该基因多态性可能与T2DM的发病有关。
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