Rahmannezhad Golzar, Mashayekhi Farideh Jalali, Goodarzi Mohammad Taghi, Rezvanfar Mohammad Reza, Sadeghi Abdorrahim
Department of Biochemistry and Genetics, Arak University of Medical Sciences, Arak, Iran.
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Gene. 2016 Apr 25;581(1):43-7. doi: 10.1016/j.gene.2016.01.026. Epub 2016 Jan 16.
It has been proposed that variants of the nuclear vitamin D receptor (VDR) gene are associated with a susceptibility to type 2 diabetes mellitus (T2DM) and gestational diabetes mellitus (GDM). Our study was aimed to evaluate a possible association between the VDR ApaI (rs7975232) and TaqI (rs731236) gene polymorphisms and susceptibility to GDM in an Iranian pregnant women population. This case-control study was performed on a population of pregnant Iranian women, including 157 GDM and 157 non-GDM subjects.VDR ApaI and TaqI polymorphisms were assessed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Our finding showed that the genotypes frequency of VDR ApaI polymorphism was significantly different between GDM and non-GDM groups (χ(2)=8.5, P=0.014). The CC genotype increased the risk of GDM as compared to the AA genotype (AA vs.CC, OR=2.996, 95% CI=1.278-7.022, P=0.012). The genotype and allele frequencies of VDR TaqI polymorphism were significantly different between GDM and non-GDM subjects (χ(2)=7.27, P=0.026, χ(2)=4.08, P=0.043 respectively). A significant protection was shown against GDM in VDR TaqI genotypes and allele (TT vs.TC, OR=0.523, 95% CI=0.23-0.84, P=0.007, TT vs. TC+CC, OR=0.546, 95% CI=0.35-0.86, P=0.009, T vs. C, OR=0.711, 95% CI=0.511-0.99, P=0.043). In conclusion, our findings show a significant association between VDR ApaI and TaqI gene polymorphisms and the GDM at the investigated loci.
有人提出，核维生素D受体（VDR）基因的变异与2型糖尿病（T2DM）和妊娠期糖尿病（GDM）的易感性有关。我们的研究旨在评估VDR ApaI（rs7975232）和TaqI（rs731236）基因多态性与伊朗孕妇人群中GDM易感性之间的可能关联。这项病例对照研究是在一群伊朗孕妇中进行的，包括157名GDM患者和157名非GDM受试者。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）评估VDR ApaI和TaqI多态性。我们的研究结果表明，GDM组和非GDM组之间VDR ApaI多态性的基因型频率存在显著差异（χ(2)=8.5，P=0.014）。与AA基因型相比，CC基因型增加了GDM的风险（AA与CC，OR=2.996，95%CI=1.278-7.022，P=0.012）。GDM组和非GDM受试者之间VDR TaqI多态性的基因型和等位基因频率存在显著差异（分别为χ(2)=7.27，P=0.026，χ(2)=4.08，P=0.043）。VDR TaqI基因型和等位基因对GDM具有显著的保护作用（TT与TC，OR=0.523，95%CI=0.23-0.84，P=0.007，TT与TC+CC，OR=0.546，95%CI=0.35-0.86，P=0.009，T与C，OR=0.711，95%CI=0.511-0.99，P=0.043）。总之，我们的研究结果表明，在研究的位点上，VDR ApaI和TaqI基因多态性与GDM之间存在显著关联。
