Jung K, Grützmann K D, Fechner C, Pergande M, Egger E
Clin Chim Acta. 1979 Oct 1;97(2-3):171-8. doi: 10.1016/0009-8981(79)90413-3.
The suitability of thirteen commercially available control sera for measuring alkaline phosphatase (EC 3.1.3.1; orthophosphoric acid monoester phosphohydrolase, ALP) activity in human serum was tested. Apart from differences in ALP activity observed in some reconstituted commercial sera, the behaviour of control materials towards experimental variables such as the nature and concentration of the substrate, pH and type of buffer (or PO4-acceptor) together with the composition of the isoenzymes present in human serum highlights the problems and difficulties if commercial materials are to be used as control sera. The half-saturation constants in control sera were in all cases smaller than those of ALP isoenzymes from bone and liver. The shape of substrate activity curves and the pH optimum in most of control sera differed from that of human serum. The discrepant kinetic data of control materials and human serum may mask or suggest changes relevant to commercial quality control serum but not to samples of human serum.
测试了13种市售对照血清用于测定人血清中碱性磷酸酶（EC 3.1.3.1；正磷酸单酯磷酸水解酶，ALP）活性的适用性。除了在一些复溶的市售血清中观察到的碱性磷酸酶活性差异外，对照物质对实验变量（如底物的性质和浓度、pH值、缓冲液类型（或磷酸受体））的反应，以及人血清中存在的同工酶组成，都凸显了如果将市售物质用作对照血清会存在的问题和困难。对照血清中的半饱和常数在所有情况下都小于来自骨骼和肝脏的碱性磷酸酶同工酶的半饱和常数。大多数对照血清中底物活性曲线的形状和最适pH值与人血清不同。对照物质和人血清不一致的动力学数据可能掩盖或暗示与市售质量控制血清相关但与人血清样本无关的变化。
