Fowler R T, Walmsley T A
Clin Chim Acta. 1978 Jul 1;87(1):159-63. doi: 10.1016/0009-8981(78)90070-0.
A study of the pH optimum of alkaline phosphatase activity in calibration and control serum preparations reveals a wide variation in pH optima. It is shown that as small an error of +/- 0.05 pH units in preparation of the buffered substrate results in an error in the order of +/- 5% in the response between the calibrating serum and patient specimens. It is recommended that a calibration serum should closely parallel the pH optimum of alkaline phosphatase in human sera and that a quality control serum containing alkaline phosphatase of alternative source to that present in the calibration serum be included with patient samples as a sensitive means of monitoring pH changes in the buffered substrate.
一项关于校准血清和对照血清制剂中碱性磷酸酶活性最适pH值的研究表明，最适pH值存在很大差异。结果显示，在制备缓冲底物时，±0.05个pH单位的微小误差会导致校准血清与患者样本之间的反应产生约±5%的误差。建议校准血清应与人类血清中碱性磷酸酶的最适pH值紧密平行，并且应将含有与校准血清中碱性磷酸酶来源不同的碱性磷酸酶的质量控制血清与患者样本一起使用，作为监测缓冲底物pH值变化的灵敏方法。
