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解析Shank3B基因敲除小鼠出生后发育阶段中Shank3在树突形态和突触功能中的作用

Deciphering the Role of Shank3 in Dendritic Morphology and Synaptic Function Across Postnatal Developmental Stages in the Shank3B KO Mouse.

作者信息

Yang Jing, Ma Guaiguai, Du Xiaohui, Xie Jinyi, Wang Mengmeng, Wang Wenting, Guo Baolin, Wu Shengxi

机构信息

Department of Basic Medicine, School of Medicine, Hangzhou City University, Hangzhou, 310015, China.

Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, Xi'an, 710032, China.

出版信息

Neurosci Bull. 2025 Apr;41(4):583-599. doi: 10.1007/s12264-024-01330-y. Epub 2024 Dec 18.

DOI:10.1007/s12264-024-01330-y
PMID:39693031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11978597/
Abstract

Autism Spectrum Disorder (ASD) is marked by early-onset neurodevelopmental anomalies, yet the temporal dynamics of genetic contributions to these processes remain insufficiently understood. This study aimed to elucidate the role of the Shank3 gene, known to be associated with monogenic causes of autism, in early developmental processes to inform the timing and mechanisms for potential interventions for ASD. Utilizing the Shank3B knockout (KO) mouse model, we examined Shank3 expression and its impact on neuronal maturation through Golgi staining for dendritic morphology and electrophysiological recordings to measure synaptic function in the anterior cingulate cortex (ACC) across different postnatal stages. Our longitudinal analysis revealed that, while Shank3B KO mice displayed normal neuronal morphology at one week postnatal, significant impairments in dendritic growth and synaptic activity emerged by two to three weeks. These findings highlight the critical developmental window during which Shank3 is essential for neuronal and synaptic maturation in the ACC.

摘要

自闭症谱系障碍（ASD）的特征是早期出现的神经发育异常，但基因对这些过程的时间动态影响仍未得到充分理解。本研究旨在阐明已知与自闭症单基因病因相关的Shank3基因在早期发育过程中的作用，为ASD潜在干预的时机和机制提供信息。利用Shank3B基因敲除（KO）小鼠模型，我们通过高尔基染色观察树突形态以及进行电生理记录以测量不同出生后阶段前扣带回皮质（ACC）的突触功能，从而检测Shank3的表达及其对神经元成熟的影响。我们的纵向分析表明，虽然Shank3B基因敲除小鼠在出生后一周时神经元形态正常，但在出生后两到三周时出现了树突生长和突触活动的显著损伤。这些发现突出了关键的发育窗口期，在此期间Shank3对于ACC中的神经元和突触成熟至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/c42dd0077f9a/12264_2024_1330_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/82179ef4bc39/12264_2024_1330_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/d63fd5433a7e/12264_2024_1330_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/03e0c9b0ff84/12264_2024_1330_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/c42dd0077f9a/12264_2024_1330_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/82179ef4bc39/12264_2024_1330_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/11d91f435bf3/12264_2024_1330_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/f24b7ae5f6ba/12264_2024_1330_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/d63fd5433a7e/12264_2024_1330_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/03e0c9b0ff84/12264_2024_1330_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99bc/11978597/c42dd0077f9a/12264_2024_1330_Fig6_HTML.jpg

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Neuron. 2024 Feb 7;112(3):441-457.e6. doi: 10.1016/j.neuron.2023.10.027. Epub 2023 Nov 21.
2
Shank3a/b isoforms regulate the susceptibility to seizures and thalamocortical development in the early postnatal period of mice.Shank3a/b 异构体调节小鼠出生后早期癫痫易感性和丘脑皮质发育。
Neurosci Res. 2023 Aug;193:13-19. doi: 10.1016/j.neures.2023.03.001. Epub 2023 Mar 5.
3
Autism Spectrum Disorder: A Review.
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JAMA. 2023 Jan 10;329(2):157-168. doi: 10.1001/jama.2022.23661.
4
Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes.利用源自单个神经嵴帽细胞的类器官模拟人类端脑发育和自闭症相关的 SHANK3 缺乏。
Nat Commun. 2022 Oct 6;13(1):5688. doi: 10.1038/s41467-022-33364-z.
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