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P460酶的硝化机制。

Nitrification Mechanisms for the P460 Enzymes.

作者信息

Siegbahn Per E M

机构信息

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm SE-106 91, Sweden.

出版信息

J Phys Chem B. 2025 Jan 9;129(1):111-116. doi: 10.1021/acs.jpcb.4c06537. Epub 2024 Dec 18.

DOI:10.1021/acs.jpcb.4c06537
PMID:39693510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11726666/
Abstract

The oxidation of hydroxylamine was studied by quantum chemical modeling. Hydroxylamine is the product of ammonia oxidation in ammonia monooxygenase. That mechanism has been studied recently by quantum chemical modeling as here. Only two enzymes can oxidize hydroxylamine, hydroxylamine oxidase and cytochrome-P460. Both employ the unusual P460-heme cofactor. In hydroxylamine oxidase, there is a covalently linked tyrosine, while in cytochrome-P460, there is a covalently linked lysine. The calculations give explanations for the experimental findings that NO is the final product in hydroxylamine oxidase, while NO is the final product in cytochrome-P460. The effect of the covalent attachments has been investigated, and reasons for their presence have been given. The methodology used, which was proven to give very good agreement with experiments for several redox enzymes, again leads to excellent agreement with experimental findings.

摘要

通过量子化学建模研究了羟胺的氧化过程。羟胺是氨单加氧酶中氨氧化的产物。最近在此处通过量子化学建模对该机制进行了研究。只有两种酶可以氧化羟胺，即羟胺氧化酶和细胞色素P460。两者都使用不寻常的P460-血红素辅因子。在羟胺氧化酶中，有一个共价连接的酪氨酸，而在细胞色素P460中，有一个共价连接的赖氨酸。计算结果对实验结果做出了解释，即羟胺氧化酶中的最终产物是NO，而细胞色素P460中的最终产物也是NO。研究了共价连接的影响，并给出了它们存在的原因。所使用的方法已被证明与几种氧化还原酶的实验结果非常吻合，再次与实验结果达成了极好的一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/a04634d14d64/jp4c06537_0011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/d16a4c984b3e/jp4c06537_0003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/7dd3ea6a5604/jp4c06537_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/6be007a6063f/jp4c06537_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/d9328a0eb84e/jp4c06537_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/711354c36a68/jp4c06537_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/989f18c22cae/jp4c06537_0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7514/11726666/a04634d14d64/jp4c06537_0011.jpg

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Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.铜和铁依赖性甲烷单加氧酶的直接甲烷氧化。
Chem Rev. 2024 Feb 14;124(3):1288-1320. doi: 10.1021/acs.chemrev.3c00727. Epub 2024 Feb 2.
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The mechanism for N activation in the E - state of nitrogenase.氮酶 E 态中氮的激活机制。
J Comput Chem. 2025 Mar 30;46(8):e70088. doi: 10.1002/jcc.70088.
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4
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