心脏肌球蛋白超松弛增加作为冬眠灰熊的一种节能机制。
Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears.
作者信息
Van der Pijl Robbert J, Ma Weikang, Lewis Christopher T A, Haar Line, Buhl Amalie, Farman Gerrie P, Rhodehamel Marcus, Jani Vivek P, Nelson O Lynne, Zhang Chengxin, Granzier Henk, Ochala Julien
机构信息
Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USA.
BioCAT, Department of Biology, Illinois Institute of Technology, Chicago, IL, USA.
出版信息
Mol Metab. 2025 Feb;92:102084. doi: 10.1016/j.molmet.2024.102084. Epub 2024 Dec 16.
AIM
The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals.
METHODS
Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics.
MAIN FINDINGS
Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability.
CONCLUSIONS
Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.
目的
本研究的目的是确定心肌肌球蛋白是否有助于冬眠哺乳动物心脏的能量保存。
方法
从活跃和冬眠的灰熊左心室分离出薄的心脏条带；并进行负载Mant-ATP追踪分析、X射线衍射和蛋白质组学分析。
主要发现
冬眠的灰熊表现出异常高比例的保存ATP的超松弛心肌肌球蛋白分子，这可能是由于磷酸化水平和杆状区域稳定性的改变。
结论
心肌肌球蛋白在冬眠期间通过调节其功能降低心脏的能量需求。
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