Rønn Marie, Nielsen Mette Olaf, Nørskov Natalja P
Department of Animal and Veterinary Sciences, Research Centre Foulum, Aarhus University Viborg, Tjele 8830, Denmark.
J Agric Food Chem. 2025 Jan 8;73(1):873-879. doi: 10.1021/acs.jafc.4c08419. Epub 2024 Dec 18.
A quantitative method was developed and validated to analyze iodoform and its potential metabolite, diiodomethane, in biological fluids from dairy cows, including rumen fluid, duodenal fluid, blood serum, milk, and urine, using liquid-liquid extraction (LLE) and GC-MS/MS. The method showed no matrix effects across different samples, recoveries of spiked samples between 70 and 120%, and relative standard deviations (RSD%) ranging from 0.7 to 14%. Inter- and intravariations in quality control samples were within 3.1-12.3%, with the highest variation noted in iodoform spiking. Normalized internal standard (IS) and matrix factor (MF) calculations confirmed the suitability of diiodomethane-d2 as an internal standard for quantifying both compounds across different matrices. These results validate the method's application for ADME (absorption, distribution, metabolism, and excretion) studies, enabling the investigation of iodoform digestion, metabolism, and excretion in dairy cattle.
开发并验证了一种定量方法，该方法采用液液萃取（LLE）和气相色谱-串联质谱（GC-MS/MS），用于分析奶牛生物体液（包括瘤胃液、十二指肠液、血清、牛奶和尿液）中的碘仿及其潜在代谢物二碘甲烷。该方法在不同样品中均未显示出基质效应，加标样品的回收率在70%至120%之间，相对标准偏差（RSD%）在0.7%至14%之间。质量控制样品的批间和批内变异在3.1%-12.3%以内，碘仿加标时变异最高。归一化内标（IS）和基质因子（MF）计算证实，二碘甲烷-d2作为内标适用于定量不同基质中的这两种化合物。这些结果验证了该方法在药物代谢动力学（吸收、分布、代谢和排泄）研究中的应用，能够研究奶牛体内碘仿的消化、代谢和排泄情况。
