Ahamad Syed Rizwan, Alhaider Abdul Qader, Raish Mohammad, Shakeel Faiyaz
Central Laboratory, Research Center, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Department of Pharmacology and Camel Biomedical Research Unit, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
Saudi J Biol Sci. 2017 Jan;24(1):23-29. doi: 10.1016/j.sjbs.2015.09.001. Epub 2015 Sep 9.
Recent studies from the author's laboratory indicated that camel urine possesses antiplatelet activity and anti-cancer activity which is not present in bovine urine. The objective of this study is to compare the volatile and elemental components of bovine and camel urine using GC-MS and ICP-MS analysis. We are interested to know the component that performs these biological activities. The freeze dried urine was dissolved in dichloromethane and then derivatization process followed by using BSTFA for GC-MS analysis. Thirty different compounds were analyzed by the derivatization process in full scan mode. For ICP-MS analysis twenty eight important elements were analyzed in both bovine and camel urine. The results of GC-MS and ICP-MS analysis showed marked difference in the urinary metabolites. GC-MS evaluation of camel urine finds a lot of products of metabolism like benzene propanoic acid derivatives, fatty acid derivatives, amino acid derivatives, sugars, prostaglandins and canavanine. Several research reports reveal the metabolomics studies on camel urine but none of them completely reported the pharmacology related metabolomics. The present data of GC-MS suggest and support the previous studies and activities related to camel urine.
作者实验室最近的研究表明，骆驼尿液具有抗血小板活性和抗癌活性，而牛尿液中不存在这些活性。本研究的目的是使用气相色谱-质谱联用仪（GC-MS）和电感耦合等离子体质谱仪（ICP-MS）分析来比较牛尿液和骆驼尿液的挥发性成分和元素成分。我们想知道发挥这些生物活性的成分是什么。将冻干的尿液溶解在二氯甲烷中，然后进行衍生化处理，接着使用N,O-双（三甲基硅基）三氟乙酰胺（BSTFA）进行GC-MS分析。通过衍生化处理在全扫描模式下分析了30种不同的化合物。对于ICP-MS分析，在牛尿液和骆驼尿液中分析了28种重要元素。GC-MS和ICP-MS分析结果显示尿液代谢物存在显著差异。对骆驼尿液的GC-MS评估发现了许多代谢产物，如苯丙酸衍生物、脂肪酸衍生物、氨基酸衍生物、糖类、前列腺素和刀豆氨酸。几项研究报告揭示了对骆驼尿液的代谢组学研究，但它们都没有完全报道与药理学相关的代谢组学。目前GC-MS的数据表明并支持了先前关于骆驼尿液的研究及活性。
