Valencia-Castillo Sandra Y, Hernández-Beza Mayte J, Powell-Cerda Irisbeth, Acosta-Cruz Erika, Rodríguez-Castillejos Guadalupe C, Siller-López Fernando, Martínez-Montoya Humberto
Universidad Libre, Seccional Pereira, Pereira, Colombia; Facultad de Medicina, Universidad de Caldas, Colombia.
Unidad Académica Multidisciplinaria Reynosa Aztlán - Universidad Autónoma de Tamaulipas, Reynosa, Mexico.
Rev Argent Microbiol. 2025 Jan-Mar;57(1):14-23. doi: 10.1016/j.ram.2024.10.006. Epub 2024 Dec 17.
Human breast milk (HBM) is a vital source of macronutrients and micronutrients that are crucial for an infant's development. Recent studies have shown that HBM contains diverse microorganisms, including bacteria, viruses, protozoa, and anaerobic fungi. Additionally, novel research has revealed that individuals with metabolic disorders, such as diabetes mellitus, are prone to dysbiosis in their gut microbiome. Our study aimed to investigate the impact of gestational diabetes mellitus (GDM) on HBM and the pair mother-infant gut microbiota. We conducted a comprehensive analysis of two groups from Pereira, Colombia: a GDM group and a non-GDM group. Each group consisted of five infants and their mothers. HBM and stool samples were collected from GDM and non-GDM mother-infant pairs. DNA was purified, and the 16S V3-V4 region was amplified and sequenced. Reads obtained were quality filtered and classified by homology according to the Ribosomal Small Subunit SILVA database. We found significant differences in the relative abundances of gut bacteria between GDM and non-GDM groups. Notably, Bifidobacterium, Serratia and Sutterella were negatively associated in women's gut with GDM. In HBM, Sutterella, Serratia and Lactococcus were found in low RA in the GDM group. Moreover, in the infants, Bifidobacterium, Lactobacillus, Sutterella, Serratia, Streptococcus, and Veillonella had a low presence in GDM. Our findings indicate that there are variations in gut bacteriome profiles between healthy women and those with GDM. These variations may impact the bacterial diversity in HBM, potentially leading to gut bacterial dysbiosis in their infants.
人乳是对婴儿发育至关重要的大量营养素和微量营养素的重要来源。最近的研究表明，人乳含有多种微生物，包括细菌、病毒、原生动物和厌氧真菌。此外，新的研究表明，患有代谢紊乱（如糖尿病）的个体肠道微生物群容易出现失调。我们的研究旨在调查妊娠期糖尿病（GDM）对人乳以及母婴肠道微生物群的影响。我们对来自哥伦比亚佩雷拉的两组人群进行了全面分析：妊娠期糖尿病组和非妊娠期糖尿病组。每组由五名婴儿及其母亲组成。从妊娠期糖尿病组和非妊娠期糖尿病组的母婴对中采集人乳和粪便样本。纯化DNA，并扩增和测序16S V3-V4区域。根据核糖体小亚基SILVA数据库对获得的读数进行质量过滤和同源分类。我们发现妊娠期糖尿病组和非妊娠期糖尿病组之间肠道细菌的相对丰度存在显著差异。值得注意的是，双歧杆菌、沙雷氏菌和萨特氏菌在患有妊娠期糖尿病的女性肠道中呈负相关。在人乳中，妊娠期糖尿病组中萨特氏菌、沙雷氏菌和乳球菌的相对丰度较低。此外，在婴儿中，双歧杆菌、乳酸杆菌、萨特氏菌、沙雷氏菌、链球菌和韦荣氏菌在妊娠期糖尿病组中的含量较低。我们的研究结果表明，健康女性和患有妊娠期糖尿病的女性之间肠道细菌群落谱存在差异。这些差异可能会影响人乳中的细菌多样性，可能导致其婴儿肠道细菌失调。
