Yu Lumin, Zhao Yuzhong, Zhang Shanpeng, Ni Weishi, Zhang Lingling, Xue Cong, Wang Peikun, Zhang Xinglin
College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China.
BMC Microbiol. 2024 Dec 19;24(1):526. doi: 10.1186/s12866-024-03694-7.
Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is currently recognized not only as a significant nosocomial pathogen but also is an emerging bacterial infection in food-producing animals, posing a critical threat to global health. However, this is a hindrance to detailed bioinformatic studies of MDR A. baumannii of chicken origin due to the lack of its complete genome sequence. Here, we report whole-genome sequencing analysis of MDR A. baumannii Y03 isolated from chickens. The Y03 genome consists of 1 circular chromosome and 4 circular plasmids, The Y03 chromosome harbors 41 antimicrobial resistance genes conferring resistance to major classes of antibiotics, including β-lactams, phenicols, macrolides, lincosamides, aminoglycosides, and nitrofurans, as well as 135 virulence factors involved in effector delivery system, immune modulation, adherence, stress survival, biofilm, exotoxin, and nutritional/metabolic factor. The in vivo infection experiments certificated that Y03 was virulent to chickens. Meanwhile, we used PCR amplification method to detect 10 antimicrobial resistance genes including abeM, adeB, adeH, adeK, bla, bla, catB9, macB, folP, and parE, as well as 14 virulence genes including lpxC, pilO, fimT, ompA, basA, bauA, gspL, csu, pgaC, plc2, tssA, tviB, bap, and vgrG. Whole-genome sequencing analysis revealed that Y03 contained 46 horizontal gene transfer elements, including 11 genomic islands, 30 transposons, and 5 prophages, as well as 518 mutations associated with reduced virulence and 44 mutations resulting in loss of pathogenicity. Furthermore, there were 22 antibiotic targets and 28 lethal mutations on the Y03 chromosome that could be used as potential targets to prevent, control, and treat infections caused by MDR A. baumannii Y03. Therefore, this study contributes to the development of strategies for the prevention, control, and treatment of A. baumannii infections and their spread in chickens.
多重耐药鲍曼不动杆菌（MDR A. baumannii）目前不仅被认为是一种重要的医院病原体，而且在产肉动物中也成为一种新出现的细菌感染源，对全球健康构成严重威胁。然而，由于缺乏来自鸡源的多重耐药鲍曼不动杆菌完整基因组序列，这对其进行详细的生物信息学研究造成了阻碍。在此，我们报告了从鸡中分离出的多重耐药鲍曼不动杆菌Y03的全基因组测序分析。Y03基因组由1条环状染色体和4个环状质粒组成，Y03染色体含有41个抗微生物耐药基因，赋予对包括β-内酰胺类、酚类、大环内酯类、林可酰胺类、氨基糖苷类和硝基呋喃类等主要抗生素类别的耐药性，以及135个毒力因子，这些毒力因子参与效应物递送系统、免疫调节、黏附、应激存活、生物膜、外毒素和营养/代谢因子等过程。体内感染实验证明Y03对鸡具有致病性。同时，我们采用PCR扩增方法检测了10个抗微生物耐药基因，包括abeM、adeB、adeH、adeK、bla、bla、catB9、macB、folP和parE，以及14个毒力基因，包括lpxC、pilO、fimT、ompA、basA、bauA、gspL、csu、pgaC、plc2、tssA、tviB、bap和vgrG。全基因组测序分析表明，Y03含有46个水平基因转移元件，包括11个基因组岛、30个转座子和5个原噬菌体，以及518个与毒力降低相关的突变和44个导致致病性丧失的突变。此外，Y03染色体上有22个抗生素靶点和28个致死性突变，可作为预防、控制和治疗由多重耐药鲍曼不动杆菌Y03引起的感染的潜在靶点。因此，本研究有助于制定预防、控制和治疗鲍曼不动杆菌感染及其在鸡群中传播的策略。
