Castro O L
Transfusion. 1985 Jan-Feb;25(1):70-2. doi: 10.1046/j.1537-2995.1985.25185116509.x.
Twelve units of red cells (RBCs) from patients with sickle cell disease were glycerolized and frozen for periods of 1 to 3 years. The freeze-thaw hemolysis (mean 11.6% +/- 2.1% SEM) and in vitro process recovery (mean 74.4% +/- 3.7% SEM) were similar to those of sickle RBCs frozen for shorter periods of time. There was a weak positive correlation between duration of frozen storage and cell losses during deglycerolization. The adenosine triphosphate and 2,3-diphosphoglycerate levels of the thawed RBCs were within an acceptable range (mean 3.4 +/- 0.2 mumol/g Hb and 18.0 +/- 0.8 mumol/g Hb, respectively). Long-term cryopreservation of sickle (Hb SS) RBCs opens the possibility of exploring autologous transfusion to treat sickle cell disease patients during anemic episodes that are not due to sickling.
对12个来自镰状细胞病患者的红细胞（RBC）单位进行甘油化处理并冷冻1至3年。冻融溶血率（平均11.6%±2.1%标准误）和体外处理回收率（平均74.4%±3.7%标准误）与冷冻较短时间的镰状红细胞相似。冷冻保存时间与去甘油化过程中的细胞损失之间存在弱正相关。解冻后红细胞的三磷酸腺苷和2,3-二磷酸甘油酸水平在可接受范围内（分别平均为3.4±0.2μmol/g血红蛋白和18.0±0.8μmol/g血红蛋白）。镰状（Hb SS）红细胞的长期冷冻保存为探索在非镰变引起的贫血发作期间对镰状细胞病患者进行自体输血治疗开辟了可能性。
