Chaplin H, Mischeaux J R, Inkster M D, Sherman L A
Transfusion. 1986 Jul-Aug;26(4):341-5. doi: 10.1046/j.1537-2995.1986.26486262741.x.
A patient with sickle cell disease and multiple alloantibodies required frozen storage of autologous red cells (RBC) to provide the RBC volume equivalent of 6 units of normal donor blood for an orthopedic surgical procedure. Eleven 600-ml donations were made at 4-week intervals, without significant change in hemoglobin concentration, hematocrit, or reticulocyte count, and with only small decreases in serum iron and ferritin concentrations. Minor modifications of a glycerolization and deglycerolization procedure described recently were used. We report in vitro recoveries of deglycerolized RBC from the 11 units following frozen storage for 27 to 391 days. Despite use of a single donor and adherence to a standardized processing protocol, considerable variation in the percent in vitro RBC recovery was observed (51-81%). In vivo survival of an aliquot following frozen storage for 27 days was identical with fresh autologous RBC (100% at 1 hr, 88% at 24 hr, one-half disappearance of 7.5 days). Autologous transfusion of RBC recovered from 7 units after storage for as long as 126 days was uneventful.
一名患有镰状细胞病且有多种同种抗体的患者，为进行骨科手术，需要冷冻保存自体红细胞（RBC），以提供相当于6单位正常供体血液的红细胞量。每隔4周进行11次600毫升的采血，血红蛋白浓度、血细胞比容或网织红细胞计数无显著变化，血清铁和铁蛋白浓度仅略有下降。采用了最近描述的甘油化和去甘油化程序的微小修改方法。我们报告了冷冻保存27至391天后，11个单位去甘油化红细胞的体外回收率。尽管使用了单一供体并遵循标准化处理方案，但观察到体外红细胞回收率百分比存在相当大的差异（51%-81%）。冷冻保存27天后的一份等分试样在体内的存活率与新鲜自体红细胞相同（1小时时为100%，24小时时为88%，半衰期为7.5天）。对保存长达126天的7个单位回收的红细胞进行自体输血，过程顺利。
