Silva Eliane P, Trentini Monalisa, Rodriguez Dunia, Kanno Alex I, Gomes Filumena M S, Valente Maria H, Trufen Carlos E M, Yamamoto Lais S, Januzzi Arthur D, Cunegundes Priscila S, Palacios Ricardo, Souza Renan P, Raw Isaías, Leite Luciana C C, Dias Waldely O
Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil.
Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Front Immunol. 2024 Dec 4;15:1472157. doi: 10.3389/fimmu.2024.1472157. eCollection 2024.
Pertussis continues to pose a significant threat despite the availability of effective vaccines. The challenge lies in the vulnerability of infants who have not yet completed their vaccination schedule and in adolescents and adults becoming potential disease carriers.
We evaluated the seroprevalence of pertussis immunity in a cohort of 1,500 healthy Brazilian volunteers. Next, we explored the potential restoration of waning pertussis immunity by administering booster doses of wP, aP or Plow (an economically viable and low reactogenic vaccine in development at Butantan) using a mouse model.
The mean anti-PT IgG levels in the Brazilian volunteers was 39.4 IU/mL. Notably, individuals ≤ 19 years exhibited higher IgG values compared to older age groups (≥ 20 y). Overall, 8.4% of the samples displayed indications of recent or current contact/infection, with IgG levels surpassing 120 IU/mL, particularly in the 15-19 years age group. IgM values were also increased in the 10-19 years age group. Potential recovery of pre-existing but waning immunity investigated in mice, showed that boosting with wP induced higher antibody titers than aP or Plow. Notably, aP and Plow boosts prompted superior effector and memory cell responses from both B and T cells. Upon challenge with , aP or Plow boost provided greater protection as compared to wP.
Pertussis appears to circulate predominantly among adolescents and young adults. Insights from the mouse model indicate that immunity can be restored with booster doses. Boosting immunity in non-targeted groups could prevent the dissemination of pertussis to infants.
尽管有有效的疫苗，但百日咳仍然构成重大威胁。挑战在于尚未完成疫苗接种计划的婴儿以及青少年和成年人成为潜在疾病携带者的脆弱性。
我们评估了1500名健康巴西志愿者队列中百日咳免疫的血清流行率。接下来，我们使用小鼠模型探索通过接种wP、aP或Plow（Butantan正在研发的一种经济可行且低反应原性的疫苗）加强剂量来恢复逐渐减弱的百日咳免疫力的可能性。
巴西志愿者的平均抗PT IgG水平为39.4 IU/mL。值得注意的是，≤19岁的个体与年龄较大的组（≥20岁）相比，IgG值更高。总体而言，8.4%的样本显示近期或当前接触/感染的迹象，IgG水平超过120 IU/mL，特别是在15 - 19岁年龄组。10 - 19岁年龄组的IgM值也有所增加。在小鼠中研究的既往但逐渐减弱的免疫力的潜在恢复情况表明，用wP加强免疫诱导的抗体滴度高于aP或Plow。值得注意的是，aP和Plow加强免疫促使B细胞和T细胞产生更好的效应细胞和记忆细胞反应。在用……攻击后，与wP相比，aP或Plow加强免疫提供了更大的保护。
百日咳似乎主要在青少年和年轻人中传播。小鼠模型的见解表明，通过加强剂量可以恢复免疫力。在非目标群体中增强免疫力可以防止百日咳传播给婴儿。
