Lundin S, Melin P, Vilhardt H
Acta Endocrinol (Copenh). 1985 Feb;108(2):179-83. doi: 10.1530/acta.0.1080179.
A sensitive and specific radioimmunoassay for 1-deamino-8-D-arginine vasopressin (DDAVP) was developed. Plasma concentrations of DDAVP after intragastric administration were determined in non-fasted rats and a dose-related increase in plasma levels was obtained. The immunoidentity of plasma DDAVP and standard was established by high performance liquid chromatography (HPLC). Correlation of biological and immunological responses of DDAVP was assessed using the antidiuretic effect in the Brattleboro (diabetes insipidus) rat.
开发了一种用于1-去氨基-8-D-精氨酸加压素（DDAVP）的灵敏且特异的放射免疫测定法。在非禁食大鼠中测定了胃内给药后DDAVP的血浆浓度，并获得了血浆水平与剂量相关的增加。通过高效液相色谱法（HPLC）确定了血浆DDAVP与标准品的免疫同一性。使用布拉特洛维（尿崩症）大鼠的抗利尿作用评估了DDAVP的生物学和免疫反应的相关性。
