İlhan Hasan, Kabakcı Dilek, Seçme Mücahit
Department of Biotechnology, Institute of Biotechnology, Ankara University, Ankara, Turkey.
Department of Animal Production and Technologies, Faculty of Applied Sciences, Muş Alparslan University, Muş, Turkey.
Med Oncol. 2024 Dec 19;42(1):32. doi: 10.1007/s12032-024-02584-2.
Thyroid cancer continues to be a notable health issue, requiring the creation of novel treatment methods to enhance patient results. The objective of this study is to investigate the potential of utilizing bee venom (BV)-loaded zeolitic imidazolate framework-8 (ZIF-8) nanoparticles as a novel strategy for specifically targeting and treating medullary thyroid cancer cells. Due to their wide surface area and configurable pore size, ZIF-8 nanoparticles are ideal for drug delivery. Bee venom's cytotoxic capabilities are used in ZIF-8 nanoparticles to target thyroid cancer cells more effectively. ZIF-8 nanoparticles containing bee venom were tested on TT medullary thyroid cancer cell lines. The effects of these nanoparticles on cell viability, proliferation, and apoptosis were investigated. IC value at 24 h for BV-ZIF-8 nanoparticles in TT medullary thyroid carcinoma cells was determined to be 17.19 µg/mL, while the IC value at 48 h was determined to be 16.39 µg/mL. It has been demonstrated that nanoparticle treatment upregulates the Bax and caspase-3 genes while downregulating the Bcl-2, CCND1, and CDK4 genes. Additionally, it was observed that oxidative stress was triggered in the nanoparticle-treated group. Furthermore, an examination of its mechanisms was conducted, with a specific emphasis on the modulation of critical signaling pathways that are implicated in the progression of cancer. In thyroid cancer cells, ZIF-8 nanoparticles infused with bee venom promote programmed cell death and impair key biological processes.
甲状腺癌仍然是一个显著的健康问题，需要开发新的治疗方法来提高患者的治疗效果。本研究的目的是探讨利用负载蜂毒（BV）的沸石咪唑酯骨架-8（ZIF-8）纳米颗粒作为一种特异性靶向和治疗甲状腺髓样癌细胞的新策略的潜力。由于ZIF-8纳米颗粒具有较大的表面积和可调节的孔径，因此是理想的药物递送载体。ZIF-8纳米颗粒利用蜂毒的细胞毒性能力更有效地靶向甲状腺癌细胞。对TT甲状腺髓样癌细胞系进行了含蜂毒的ZIF-8纳米颗粒测试。研究了这些纳米颗粒对细胞活力、增殖和凋亡的影响。TT甲状腺髓样癌细胞中BV-ZIF-8纳米颗粒在24小时时的IC值确定为17.19μg/mL，而48小时时的IC值确定为16.39μg/mL。已证明纳米颗粒处理上调Bax和caspase-3基因，同时下调Bcl-2、CCND1和CDK4基因。此外，观察到纳米颗粒处理组引发了氧化应激。此外，对其机制进行了研究，特别强调了与癌症进展相关的关键信号通路的调节。在甲状腺癌细胞中，注入蜂毒的ZIF-8纳米颗粒促进程序性细胞死亡并损害关键生物学过程。
