Kakinuma Yoshihiko
Department of Bioregulatory Science, Nippon Medical School, Tokyo, Japan.
Methods Mol Biol. 2025;2894:1-6. doi: 10.1007/978-1-0716-4342-6_1.
Isolation of primary cardiomyocytes can be performed for studies from fetuses and neonates. Compared with rat neonatal cardiomyocytes, mouse neonatal cardiomyocytes are sensitive to enzyme digestion. Therefore, they need to optimize a concentration of collagenase and a time point to collect the heart after birth. In addition, neonatal cardiomyocytes have unique characteristics to be recognized although the preparation steps are not complicated. Therefore, depending on a researcher's interest and aim, they consider which sources of cardiomyocytes are suitable and convenient, for example, a rat or mouse, as well as a fetus and neonate.
可以从胎儿和新生儿中分离出原代心肌细胞用于研究。与大鼠新生心肌细胞相比，小鼠新生心肌细胞对酶消化敏感。因此，需要优化胶原酶浓度和出生后采集心脏的时间点。此外，尽管制备步骤并不复杂，但新生心肌细胞具有一些独特的特性需要了解。因此，根据研究人员的兴趣和目的，他们会考虑哪种心肌细胞来源合适且方便，例如大鼠或小鼠，以及胎儿和新生儿。
