Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital.

OBJECTIVE

To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP).

MATERIAL AND METHODS

Sixty patients from the Orthodontics department of Subharti Dental College and Hospital (Meerut, India) were divided into two groups: Group 1 (Class II div. 1 malocclusion) and Group 2 (Class II div. 2 malocclusion). Then, 3 mL of blood was collected from each participant. DNA extraction was done, and Sanger Sequencing was performed from extracted DNA samples.

RESULTS

A statistically significant difference was found in the distribution of alleles among Class II div. 1 and Class II div. 2 malocclusions. The homozygous GG allele was the most prevalent among Class II div. 1 patients (76.7%), while the heterozygous AG allele was the most prevalent among Class II div. 2 patients (53.5%). Since GG was the most prevalent allele, it was used as a reference, and AA/AG were compared with GG to confirm the association. The results showed that individuals with the AG genotype seemed to be more susceptible to the development of skeletal Class II div. 2 malocclusion.

CONCLUSION

The homozygous GG allele was the most prevalent among Class II div. 1 patients, while the heterozygous AG allele was the most prevalent among Class II div. 2 patients, suggesting that there could be a difference between the genetic association of both malocclusions.