Shukla Monika, Reddy Munish, Singh Kritanjali, Saini Ruchi, Raghav Pradeep, Nasser Kaynat, Sharma Nupur, Kamrani Aastha, Rafique Shehla, Shair Tanjula
Subharti Dental College, Department of Orthodontics and Dentofacial Orthopedics (Uttar Pradesh, India).
Subharti Medical College & Hospital, Central Research and Incubation Center (Uttar Pradesh, India).
Dental Press J Orthod. 2024 Dec 16;29(6):e2424128. doi: 10.1590/2177-6709.29.6.e2424128.oar. eCollection 2024.
To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP).
Sixty patients from the Orthodontics department of Subharti Dental College and Hospital (Meerut, India) were divided into two groups: Group 1 (Class II div. 1 malocclusion) and Group 2 (Class II div. 2 malocclusion). Then, 3 mL of blood was collected from each participant. DNA extraction was done, and Sanger Sequencing was performed from extracted DNA samples.
A statistically significant difference was found in the distribution of alleles among Class II div. 1 and Class II div. 2 malocclusions. The homozygous GG allele was the most prevalent among Class II div. 1 patients (76.7%), while the heterozygous AG allele was the most prevalent among Class II div. 2 patients (53.5%). Since GG was the most prevalent allele, it was used as a reference, and AA/AG were compared with GG to confirm the association. The results showed that individuals with the AG genotype seemed to be more susceptible to the development of skeletal Class II div. 2 malocclusion.
The homozygous GG allele was the most prevalent among Class II div. 1 patients, while the heterozygous AG allele was the most prevalent among Class II div. 2 patients, suggesting that there could be a difference between the genetic association of both malocclusions.
使用PAX9（rs8004560）基因单核苷酸多态性（SNP）评估安氏II类1分类（1分类）与2分类（2分类）错牙合畸形之间的遗传关联是否存在差异。
将来自印度密鲁特苏巴蒂牙科学院和医院正畸科的60例患者分为两组：第1组（安氏II类1分类错牙合畸形）和第2组（安氏II类2分类错牙合畸形）。然后，从每位参与者采集3毫升血液。进行DNA提取，并对提取的DNA样本进行桑格测序。
发现安氏II类1分类和安氏II类2分类错牙合畸形的等位基因分布存在统计学显著差异。纯合子GG等位基因在安氏II类1分类患者中最为常见（76.7%），而异合子AG等位基因在安氏II类2分类患者中最为常见（53.5%）。由于GG是最常见的等位基因，将其用作参照，并将AA/AG与GG进行比较以确认关联。结果显示，AG基因型个体似乎更容易患骨性安氏II类2分类错牙合畸形。
纯合子GG等位基因在安氏II类1分类患者中最为常见，而异合子AG等位基因在安氏II类2分类患者中最为常见，这表明两种错牙合畸形的遗传关联可能存在差异。
