Brackett R G, Cole G C, Green T J, Jacobs R L
Bull World Health Organ. 1979;57 Suppl 1(Suppl):33-6.
A system with continuous flow of medium that uses disposable tissue culture flasks for the in vitro cultivation of P. falciparum is described. This scaled-up modification of the Trager and Jensen method gives merozoite yields sufficient for experimental vaccine studies in animals. A method of increasing the yield of merozoites by decreasing the pH of the medium is presented.
描述了一种使用一次性组织培养瓶进行恶性疟原虫体外培养的、具有连续培养基流动的系统。这种对特拉格和詹森方法的放大改进可产生足以用于动物实验性疫苗研究的裂殖子产量。还介绍了一种通过降低培养基pH值来提高裂殖子产量的方法。