Reber-Liske R
Acta Trop. 1983 Mar;40(1):39-43.
The in vitro cultivation of Plasmodium falciparum requires at least daily changes of medium (Trager and Jensen, 1976). Addition of 50 mg per litre of hypoxanthine to medium RPMI 1640 permits to postpone the change of medium for up to 72 hours. A single subculture step is required to remove unlabelled hypoxanthine prior to the use of the cultured material in 3H-hypoxanthine incorporation assays. The method constitutes a considerable saving of time and medium.
恶性疟原虫的体外培养至少需要每天更换培养基（Trager和Jensen，1976年）。在RPMI 1640培养基中添加每升50毫克的次黄嘌呤可将更换培养基的时间推迟至72小时。在将培养物用于3H-次黄嘌呤掺入试验之前，需要进行一次传代培养步骤以去除未标记的次黄嘌呤。该方法可大量节省时间和培养基。
