Qian Junqing, Shi Bobo, Li Qian, Gou Lihong, Zhao Changyan, Huang Aomei
College of Pharmaceutical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
College of Pharmaceutical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
Food Chem. 2025 Mar 15;468:142231. doi: 10.1016/j.foodchem.2024.142231. Epub 2024 Nov 29.
Biological and interfacial imprinting both were effective methods to improve the catalytic performance of lipase in organic solvent. Bioimprinting combined with interfacial activation of lipase was investigated for obtaining imprinted lipase with excellent catalytic performance. Enzymatic synthesis of sucrose-6-acetate in organic solvents to test the performance of imprinted lipase immobilized by adsorbing on macroporous resin. Lipase imprinted by substrate analog (oleic acid) then immobilization catalyzed the synthesis of sucrose-6-acetate with a 1.7 times increase in sucrose esterification rate compared to unimprinted immobilized lipase and a selectivity toward sucrose-6-acetate of 91.7 %. The sucrose esterification rate of reaction catalyzed by immobilized lipase was increased to 2.0 times with addition of sorbitol after imprinting by oleic acid. Imprinting by oleic acid and interfacial effect generated by oleic acid with sorbitol both induced zymoprotein secondary structure change that were conducive to enhance catalytic activity and stability of lipase in organic solvent.
生物印迹和界面印迹都是提高脂肪酶在有机溶剂中催化性能的有效方法。研究了生物印迹与脂肪酶界面活化相结合的方法，以获得具有优异催化性能的印迹脂肪酶。在有机溶剂中进行蔗糖-6-乙酸酯的酶促合成，以测试通过吸附在大孔树脂上固定化的印迹脂肪酶的性能。用底物类似物（油酸）印迹脂肪酶，然后固定化，与未印迹的固定化脂肪酶相比，催化蔗糖-6-乙酸酯的合成，蔗糖酯化率提高了1.7倍，对蔗糖-6-乙酸酯的选择性为91.7%。用油酸印迹后，加入山梨醇，固定化脂肪酶催化反应的蔗糖酯化率提高到2.0倍。油酸印迹和油酸与山梨醇产生的界面效应均诱导了酶蛋白二级结构的变化，有利于提高脂肪酶在有机溶剂中的催化活性和稳定性。
