Guan Guoqiang, Zhang Yuyang, Qian Jingya, Wang Feng, Qu Liang, Zou Bin
School of Food and Biological Engineering, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, China.
School of Food and Biological Engineering, Wuhu Institute of Technology, Wuhu 241003, China.
Materials (Basel). 2025 May 25;18(11):2476. doi: 10.3390/ma18112476.
This study aims to delve into the application potential of immobilized lipases in the catalytic synthesis of isoamyl acetate. Through a comparative analysis of various immobilization methods, including physical adsorption, encapsulation, covalent binding, and crosslinking, along with the utilization of nanomaterials, such as magnetic nanoparticles, mesoporous silica SBA-15, and covalent organic frameworks (COFs) as carriers, the study systematically evaluates their enhancing effects on lipase catalytic performance. Additionally, solvent engineering strategies, encompassing the introduction of organic solvents, supercritical fluids, ionic liquids, and deep eutectic solvents, are employed to intensify the enzymatic catalytic process. These approaches effectively improve mass transfer efficiency, activate enzyme molecules, and safeguard enzyme structural stability, thereby significantly elevating the synthesis efficiency and yield of isoamyl acetate. Consequently, this research provides solid scientific rationale and technical support for the industrial production of flavor ester compounds.
本研究旨在深入探讨固定化脂肪酶在乙酸异戊酯催化合成中的应用潜力。通过对包括物理吸附、包封、共价结合和交联在内的各种固定化方法，以及利用磁性纳米颗粒、介孔二氧化硅SBA - 15和共价有机框架（COFs）等纳米材料作为载体进行比较分析，该研究系统地评估了它们对脂肪酶催化性能的增强作用。此外，采用包括引入有机溶剂、超临界流体、离子液体和深共熔溶剂在内的溶剂工程策略来强化酶催化过程。这些方法有效地提高了传质效率，激活了酶分子，并保障了酶的结构稳定性，从而显著提高了乙酸异戊酯的合成效率和产率。因此，本研究为风味酯类化合物的工业化生产提供了坚实的科学依据和技术支持。
