Wu Mangang, Xu Yuyu, Gu Chen, Wang Jiahao, Wang Qingling, Yin Peipei, Zhu Tianhao, Yin Qing, Zhao Xinxin, Jin Duxin, Liu Rui, Ge Qingfeng, Yu Hai
College of Food Science and Engineering, Yangzhou University, Yangzhou, China.
J Sci Food Agric. 2025 Apr;105(6):3397-3405. doi: 10.1002/jsfa.14101. Epub 2024 Dec 29.
Pickering emulsions prepared with octenyl succinic anhydride-modified starch (OSAS) show significant promise as replacements for animal fat. However, the underlying mechanism of incorporating an OSAS-based Pickering emulsion into a myofibrillar protein (MP) gel and its impact on the gel properties remain poorly understood. In this study, the effects of OSAS at varying concentrations (0-10.0 g kg) on the stability of Pickering emulsion and the resultant gel properties of MP were investigated.
Emulsion stability was assessed using a stability analyzer, revealing a significant enhancement with increasing OSAS concentration. Compared with MP gel, the incorporation of the OSAS-based Pickering emulsion markedly improved the texture of the composite gels, increasing the gel hardness from 0.28 to 0.66 N. Moreover, water-holding capacity of composite gels rose from 28.5% to 61.2%, with a notable increase in immobilized water and a decrease in mobilized water. Rheological analysis revealed that the interactions of modified starch with MP and water molecules bolstered the elastic modulus of the gels. Additionally, the presence of OSAS-stabilized emulsions led to reduced surface hydrophobicity and sulfhydryl content of proteins in the gels, while partially inhibiting protein oxidation.
OSAS, notably at a high concentration, improved the physical stability of Pickering emulsion and the properties of MP gel. This research provides fundamental insights for the development of high-quality emulsified meat products. © 2024 Society of Chemical Industry.
用辛烯基琥珀酸酐改性淀粉(OSAS)制备的Pickering乳液作为动物脂肪的替代品显示出巨大潜力。然而,将基于OSAS的Pickering乳液加入肌原纤维蛋白(MP)凝胶的潜在机制及其对凝胶特性的影响仍知之甚少。在本研究中,研究了不同浓度(0 - 10.0 g/kg)的OSAS对Pickering乳液稳定性以及MP所得凝胶特性的影响。
使用稳定性分析仪评估乳液稳定性,结果表明随着OSAS浓度的增加稳定性显著增强。与MP凝胶相比,加入基于OSAS的Pickering乳液显著改善了复合凝胶的质地,使凝胶硬度从0.28 N增加到0.66 N。此外,复合凝胶的持水能力从28.5%提高到61.2%,固定水显著增加,游离水减少。流变学分析表明,改性淀粉与MP和水分子的相互作用增强了凝胶的弹性模量。此外,OSAS稳定乳液的存在导致凝胶中蛋白质的表面疏水性和巯基含量降低,同时部分抑制了蛋白质氧化。
OSAS,尤其是高浓度时,改善了Pickering乳液的物理稳定性和MP凝胶的特性。本研究为开发高品质乳化肉制品提供了基础见解。© 2024化学工业协会。