Lin Xiao-Yu, Wei Ji-Chang, Zhao Yi-Hang, Xu Ran, Tan Xin-Ru, Huang Xue-Mei, Wang Peng-Long
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
Zhongguo Zhong Yao Za Zhi. 2024 Sep;49(18):4948-4956. doi: 10.19540/j.cnki.cjcmm.20240617.302.
This study employed supramolecular chemistry of traditional Chinese medicine to compare the material basis between different decocting methods(co-decocting and single decocting) and explained the chemical basis of Rhei Radix et Rhizoma-Coptidis Rhizoma in alleviating the disadvantage of bitter and cold medicines damaging the stomach. The spectroscopic differences between the co-decoction and single decoctions were characterized by ultraviolet-visible absorption spectrum, infrared spectrum, and circular dichroism spectrum. Five characteristic alkaloids, such as berberine, were selected as the indicators to compare the sample solubility and dissolution of medicinal components between the co-decoction and single decoctions by high performance liquid chromatography. The chemical components and their total content were similar between the co-decoction and single decoctions of Rhei Radix et Rhizoma and Coptidis Rhizoma. The medicinal components in the co-decoction interacted with each other to form supramolecular parts. The dissolution and release of supramolecular parts in the aqueous solution was slow, which explained the lower component content in the co-decoction than that in single decoctions. In addition, the adsorption of medicinal components by the residue of decoction pieces during decocting was also a factor causing the difference of material basis between different decocting methods. This study compared the material basis between the co-decoction and single decoctions of Rhei Radix et Rhizoma and Coptidis Rhizoma from the dynamic changes of components, which provided a basis further explaining the scientific connotation of the compatibility of the two medicines in reducing the bitter and cold medicinal natures.
本研究运用中药超分子化学比较不同煎煮方法（合煎与单煎）之间的物质基础，并阐释大黄 - 黄连在减轻苦寒药物伤胃弊端方面的化学依据。通过紫外 - 可见吸收光谱、红外光谱和圆二色光谱对合煎液与单煎液之间的光谱差异进行表征。选取小檗碱等5种特征生物碱作为指标，采用高效液相色谱法比较合煎液与单煎液中药物成分的样品溶解度和溶出情况。大黄与黄连合煎液和单煎液中的化学成分及其总量相似。合煎液中的药物成分相互作用形成超分子部件。超分子部件在水溶液中的溶解和释放缓慢，这解释了合煎液中成分含量低于单煎液的原因。此外，煎煮过程中饮片残渣对药物成分的吸附也是导致不同煎煮方法物质基础差异的一个因素。本研究从成分动态变化方面比较了大黄与黄连合煎液和单煎液的物质基础，为进一步阐释二者配伍减其苦寒药性的科学内涵提供了依据。
