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基于智能感官技术和多元统计分析的黄芪蜂蜜加工度快速识别

[Rapid identification of honey-processing degree of Astragali Radix based on intelligent sensory technology and multivariate statistical analyses].

作者信息

Wu Yi, Yu He-Rong, Nie Xin-Ru, Guo Qiang, Shen Zhe-Yuan, Zhang Jiu-Ba, Li Ming-Xuan, Mei Xi, Guo Zhi-Jun, Gao Bo, Mao Chun-Qin, Lu Tu-Lin, Zhao Xiao-Li

机构信息

School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.

Anhui Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule Huaibei 235000, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2024 Oct;49(20):5451-5459. doi: 10.19540/j.cnki.cjcmm.20240717.301.

Abstract

To analyze the differences and dynamic changes in the color, odor, and major chemical components of Astragali Radix during honey processing, this study used a CM-5 spectrophotometer and Hercules NEO ultra-fast gas phase electronic nose for analysis. High performance liquid chromatography was employed to determine the content of calycosin, calycosin-7-glucoside, formononetin, and ononin in the Astragali Radix decoction pieces processed with honey to different degrees. Multivariate statistical analyses including partial least squares-discriminant analysis(PLS-DA), orthogonal partial least squares-discriminant analysis(OPLS-DA), differential factor analysis(DFA), and Bayesian discriminant analysis were adopted to differentiate the Astragali Radix decoction pieces processed with honey to different degrees and evaluate the correlations between visual characteristics and chemical composition. The results showed that chromatic values c*, b, and L~ were the main color difference variables distinguishing raw and honey-processed Astragali Radix decoction pieces. The reference ranges for the chromatic values of raw and honey-processed Astragali Radix decoction pieces were determined based on these key color parameters. A total of 18 odor components were identified in Astragali Radix during the honey-processing process, with cyclohexane and dimethyl sulfoxide identified as odor markers for distinguishing Astragali Radix decoction pieces with different honey-processing degrees. The content of calycosin had a significant correlation with that of 2-methylfuran and delta-nonalactone, two odor components. This study successfully and rapidly differentiated the Astragali Radix decoction pieces processed with honey to different degrees, providing a reference for the quality control and the quality evaluation of Astragali Radix decoction pieces based on the appearance traits during the process of honey processing.

摘要

为分析蜜制过程中黄芪颜色、气味及主要化学成分的差异和动态变化,本研究采用CM - 5分光光度计和Hercules NEO超快速气相电子鼻进行分析。采用高效液相色谱法测定不同程度蜜制黄芪饮片中山柰酚、山柰酚 - 7 - 葡萄糖苷、芒柄花素和芒柄花苷的含量。采用偏最小二乘判别分析(PLS - DA)、正交偏最小二乘判别分析(OPLS - DA)、差异因子分析(DFA)和贝叶斯判别分析等多元统计分析方法,对不同程度蜜制的黄芪饮片进行区分,并评估外观特征与化学成分之间的相关性。结果表明,色度值c*、b*和L~*是区分生黄芪饮片和蜜制黄芪饮片的主要色差变量。基于这些关键颜色参数确定了生黄芪饮片和蜜制黄芪饮片色度值的参考范围。在黄芪蜜制过程中共鉴定出18种气味成分,其中环己烷和二甲基亚砜被确定为区分不同蜜制程度黄芪饮片的气味标志物。山柰酚含量与两种气味成分2 - 甲基呋喃和δ - 壬内酯含量具有显著相关性。本研究成功快速地区分了不同程度蜜制的黄芪饮片,为蜜制过程中基于外观性状的黄芪饮片质量控制和质量评价提供了参考。

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