Yu Chun-Xia, Kong Xiang-He, Ren Guang-Xi, Xu Yu-Bin, Jiang Dan, Liu Chun-Sheng
Henan Provincial Center for Drug Evaluation and Inspection Zhengzhou 450000, China.
Beijing University of Chinese Medicine Beijing 102488, China.
Zhongguo Zhong Yao Za Zhi. 2024 Oct;49(20):5497-5504. doi: 10.19540/j.cnki.cjcmm.20240713.103.
Inductively coupled plasma mass spectrometry was employed to determine the content of 25 inorganic elements in Bambusae Concretio Silicea, and the elemental fingerprint was established according to the element content. SPSS 20.0 and SIMCA 14.1 were used for chemometric analysis. A total of 25 elements were detected in Bambusae Concretio Silicea, including boron(B), sodium(Na), magnesium(Mg), aluminum(Al), potassium(K), calcium(Ca), scandium(Sc), vanadium(V), chromium(Cr), manganese(Mn), iron(Fe), cobalt(Co), nickel(Ni), copper(Cu), zinc(Zn), gallium(Ga), arsenic(As), selenium(Se), zirconium(Zr), molybdenum(Mo), cadmium(Cd), indium(In), barium(Ba), plumbum(Pb), and bismuth(Bi), among which 13 elements were quantitative. The top three elements in terms of average content were K>Fe>Na, and the content of B, Na, Al, Ca, Fe, and Ba varied in different batches of Bambusae Concretio Silicea samples from different origins. The correlation coefficient between Ca and Mg was the highest, followed by that between Mn and Zn, both of which showed significant positive correlations. Therefore, it was hypothesized that there was a synergistic incremental relationship between Ca and Mg as well as between Mn and Zn. According to the results of principal component analysis, Fe, As, Ni, Cu, Mn, Na, Mg, Ca, Al, Ba, and B were the main elements of Bambusae Concretio Silicea. According to the results of orthogonal partial least squares-discriminant analysis, Cu, As, and Mn were the common differential elements of three origins, which suggested that the quantitative identification of Cu, As, and Mn could roughly distinguish the three batches of Bambusae Concretio Silicea samples. This study analyzed the elements and their content in different batches of Bambusae Concretio Silicea samples, aiming to provide theoretical support for exploring the pharmacodynamic material basis and mechanism of Bambusae Concretio Silicea and lay a foundation for the quality evaluation, safe application, and resource development and utilization of Bambusae Concretio Silicea.
采用电感耦合等离子体质谱法测定了竹黄中25种无机元素的含量，并根据元素含量建立了元素指纹图谱。使用SPSS 20.0和SIMCA 14.1进行化学计量学分析。在竹黄中共检测到25种元素，包括硼（B）、钠（Na）、镁（Mg）、铝（Al）、钾（K）、钙（Ca）、钪（Sc）、钒（V）、铬（Cr）、锰（Mn）、铁（Fe）、钴（Co）、镍（Ni）、铜（Cu）、锌（Zn）、镓（Ga）、砷（As）、硒（Se）、锆（Zr）、钼（Mo）、镉（Cd）、铟（In）、钡（Ba）、铅（Pb）和铋（Bi），其中13种元素为定量元素。按平均含量排序前三位的元素为K＞Fe＞Na，不同产地的不同批次竹黄样品中B、Na、Al、Ca、Fe和Ba的含量存在差异。Ca与Mg之间的相关系数最高，其次是Mn与Zn之间的相关系数，二者均呈显著正相关。因此，推测Ca与Mg之间以及Mn与Zn之间存在协同递增关系。根据主成分分析结果，Fe、As、Ni、Cu、Mn、Na、Mg、Ca、Al、Ba和B是竹黄的主要元素。根据正交偏最小二乘法判别分析结果，Cu、As和Mn是三个产地的共同差异元素，这表明对Cu、As和Mn进行定量鉴定可大致区分三批竹黄样品。本研究分析了不同批次竹黄样品中的元素及其含量，旨在为探索竹黄的药效物质基础及作用机制提供理论支持，并为竹黄的质量评价、安全应用以及资源开发利用奠定基础。
