Lei Shikang, Li Guangguang, Jiang Ding, Yuan Fanchong, Zheng Yansong, Cao Bihao, Zhang Hua
Guangzhou Academy of Agricultural Sciences, Guangzhou, 510335, China.
College of Horticulture, South China Agricultural University, Guangzhou, 510642, China.
BMC Plant Biol. 2024 Dec 19;24(1):1192. doi: 10.1186/s12870-024-05807-w.
The modification of protein substrates by small ubiquitin-related modifier (SUMO) plays a vital role in plants subjected to biotic and abiotic stresses. However, its role in the stress responses of Brassica plants remains poorly understood.
A genome-wide analysis revealed the presence of 30 SUMOylation genes in the Caixin genome. These results demonstrated that the Caixin genome contains all the necessary components for SUMOylation. Analysis of the cis-acting elements revealed that the promoters of SUMOylation genes presented diverse combinations of developmental and stress-related cis-regulatory elements. The RNA-seq data indicated that 23 SUMOylation genes presented relatively high expression levels under normal conditions and exhibited a notable decrease in expression following Pectobacterium carotovorum subsp. carotovorum (Pcc) infection. Additionally, dynamic alterations in SUMO conjugates were observed in response to Pcc infection.
The Caixin genome contains genes involved in SUMOylation. The majority of these genes presented multiple copies, and analyses of their transcription and protein profiles indicate that they may play a role in the response to Pcc infection.
小泛素相关修饰物（SUMO）对蛋白质底物的修饰在遭受生物和非生物胁迫的植物中起着至关重要的作用。然而，其在芸苔属植物胁迫反应中的作用仍知之甚少。
全基因组分析揭示了菜心中存在30个SUMO化基因。这些结果表明，菜心基因组包含SUMO化所需的所有必要成分。对顺式作用元件的分析表明，SUMO化基因的启动子呈现出发育和胁迫相关顺式调控元件的多种组合。RNA测序数据表明，23个SUMO化基因在正常条件下表达水平相对较高，而在胡萝卜软腐果胶杆菌胡萝卜亚种（Pcc）感染后表达显著下降。此外，观察到SUMO缀合物响应Pcc感染发生动态变化。
菜心基因组包含参与SUMO化的基因。这些基因大多数有多个拷贝，对其转录和蛋白质谱的分析表明，它们可能在对Pcc感染的反应中发挥作用。
