Luo Chenglin, Liu Xinliang, Zheng Yongjie, Dai Xiaoying, Tang Xinglin, Zhang Ting, Zhang Xuhui
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing, Jiangsu, 210037, P.R. China.
Camphor Engineering and Technology Research Centre of National Forestry and Grassland Administration, Jiangxi Academy of Forestry, Nanchang, 330032, China.
BMC Plant Biol. 2024 Dec 19;24(1):1217. doi: 10.1186/s12870-024-05941-5.
Cinnamomum parthenoxylon is a significant essential oil plant in southern China, however, the challenge of rooting cuttings poses a hindrance to its development and widespread cultivation. Adventitious root (AR) formation is a vital mechanism for plants to acclimate to environmental changes, yet the precise regulatory mechanisms governing this process remain largely unknown. This study investigated the morphological, physiological, and transcriptomic alterations during AR formation in C. parthenoxylon. Our findings revealed that the AR in C. parthenoxylon originated from callus tissue. Nutrients, enzymes, and plant hormones exerted crucial functions in cutting propagation, with some gradually shifting roles in the rooting process until the pivotal stage of root primordium initiation. Analysis of differentially expressed genes (DEGs) highlighted the significance of auxin, ethylene, and plant wound signaling pathways in regulating AR. Furthermore, 14 hub genes were identified through protein-protein interaction (PPI) networks, shedding light on key molecular players. Understanding the dynamics involved in AR formation enhances our comprehension of the regulatory mechanisms, offering insights for optimizing cutting treatment methods.
樟树是中国南方重要的精油植物，然而，扦插生根的难题阻碍了其发展和广泛种植。不定根（AR）的形成是植物适应环境变化的重要机制，但调控这一过程的精确机制仍 largely 未知。本研究调查了樟树不定根形成过程中的形态、生理和转录组变化。我们的研究结果表明，樟树的不定根起源于愈伤组织。营养物质、酶和植物激素在扦插繁殖中发挥着关键作用，其中一些在生根过程中作用逐渐转变，直至根原基起始的关键阶段。差异表达基因（DEG）分析突出了生长素、乙烯和植物伤口信号通路在调控不定根中的重要性。此外，通过蛋白质 - 蛋白质相互作用（PPI）网络鉴定出 14 个枢纽基因，揭示了关键分子参与者。了解不定根形成所涉及的动态过程增强了我们对调控机制的理解，为优化扦插处理方法提供了见解。
