利用RAD-seq技术构建红麻第一朵花节位的高密度遗传图谱并进行QTL分析。
High-density genetic map construction and QTL analysis of the first flower node in kenaf using RAD-seq.
作者信息
Li Hui, Chen Anguo, Tang Huijuan, Luan Mingbao
机构信息
College of Life and Environmental Sciences, Hunan University of Arts and Science, No. 3150 Dongting Avenue, Changde, Hunan, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, 348 West Xianjiahu Road, Changsha, Hunan, China.
出版信息
BMC Plant Biol. 2024 Dec 19;24(1):1191. doi: 10.1186/s12870-024-05907-7.
BACKGROUND
In kenaf (Hibiscus cannabinus L.) which is an important natural fiber crop, the first flower node is closely linked to fiber yield. However, the genetic mechanisms controlling the first flower node remain poorly understood.
RESULTS
In this study, we constructed a high-density genetic linkage map using Restriction-site associated DNA sequencing (RAD-seq) technology based on 148 individual hybrid kenaf progeny. The map contained 3418 bin markers unevenly on 18 linkage groups and spanned a total of 864.607 cM with an average distance of 0.253 cM. Based on the genetic map, two major QTLs, FFN6.1 and FFN6.2, were detected on LG6 and were strongly associated with the first flower node. Based on annotation information in the GO, KEGG and NR public databases, 373 candidate genes were predicted in the FFN6.1 and FFN6.2 regions. Finally, using RT-qPCR, three genes related to the first flower node were identified.
CONCLUSION
The study provides a good foundation for understanding the molecular mechanisms of the first flower node trait in kenaf.
背景
红麻（Hibiscus cannabinus L.）是一种重要的天然纤维作物，其第一朵花着生节位与纤维产量密切相关。然而，控制第一朵花着生节位的遗传机制仍知之甚少。
结果
在本研究中，我们基于148个红麻杂交后代个体，利用限制性位点关联DNA测序（RAD-seq）技术构建了一张高密度遗传连锁图谱。该图谱在18个连锁群上不均匀地分布着3418个bin标记，图谱总长864.607 cM，平均间距为0.253 cM。基于该遗传图谱，在LG6上检测到两个主要的QTL，即FFN6.1和FFN6.2，它们与第一朵花着生节位显著相关。基于GO、KEGG和NR公共数据库中的注释信息，在FFN6.1和FFN6.2区域预测到373个候选基因。最后，通过RT-qPCR鉴定出3个与第一朵花着生节位相关的基因。
结论
该研究为理解红麻第一朵花着生节位性状的分子机制提供了良好的基础。
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