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阐明负责优化宿主细胞裂解的噬菌体蛋白的分子功能。

Elucidation of molecular function of phage protein responsible for optimization of host cell lysis.

作者信息

Kim Jinwoo, Kim Joonbeom, Ryu Sangryeol

机构信息

Department of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.

Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.

出版信息

BMC Microbiol. 2024 Dec 19;24(1):532. doi: 10.1186/s12866-024-03684-9.

DOI:10.1186/s12866-024-03684-9
PMID:39702038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11657625/
Abstract

BACKGROUND

Bacteriophages (or phages) replicate by utilizing bacterial resources and destroy their host cells at the end of the replication cycle. Phages employ multiple proteins to optimize host cell lysis, thereby maximizing the production of phage particles. However, elucidating the entire lysis process is challenging due to the abundance of uncharacterized genes in the phage genome.

RESULTS

In this study, we identified a gene orf52 from BSPM4 phage genome that showed antibacterial activity in Salmonella. Investigation of physiological role of ORF52 in the phage replication revealed that ORF52 could modulate the holin function to fine-tune a cell lysis, providing replication advantages to phages under high phage population density.

CONCLUSIONS

We concluded that ORF52 may optimize phage replication by modulating the timing of phage-mediated cell lysis. This study provides a unique example of a phage protein involved in fine-tuning lysis timing.

摘要

背景

噬菌体通过利用细菌资源进行复制，并在复制周期结束时破坏其宿主细胞。噬菌体利用多种蛋白质来优化宿主细胞裂解，从而使噬菌体颗粒的产量最大化。然而，由于噬菌体基因组中存在大量未表征的基因，阐明整个裂解过程具有挑战性。

结果

在本研究中，我们从BSPM4噬菌体基因组中鉴定出一个基因orf52，其在沙门氏菌中表现出抗菌活性。对ORF52在噬菌体复制中的生理作用的研究表明，ORF52可以调节孔蛋白功能以微调细胞裂解，在高噬菌体群体密度下为噬菌体提供复制优势。

结论

我们得出结论，ORF52可能通过调节噬菌体介导的细胞裂解时间来优化噬菌体复制。本研究提供了一个参与微调裂解时间的噬菌体蛋白的独特例子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/42a1039b11ac/12866_2024_3684_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/65c18fd4c812/12866_2024_3684_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/eef910daef75/12866_2024_3684_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/30c2c5100c6e/12866_2024_3684_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/11d6623be6cc/12866_2024_3684_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/37f54bc26bd6/12866_2024_3684_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/0f9ba55de274/12866_2024_3684_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/42a1039b11ac/12866_2024_3684_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/65c18fd4c812/12866_2024_3684_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/eef910daef75/12866_2024_3684_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/30c2c5100c6e/12866_2024_3684_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/11d6623be6cc/12866_2024_3684_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/37f54bc26bd6/12866_2024_3684_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/0f9ba55de274/12866_2024_3684_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84e1/11657625/42a1039b11ac/12866_2024_3684_Fig7_HTML.jpg

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本文引用的文献

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Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria.一种靶向革兰氏阴性菌的新型噬菌体溶菌酶的鉴定及体外特性研究
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