Rieger R, Kaderli S, Boulocher C
Université de Lyon, VetAgro Sup, UPSP ICE 2021.A104, Marcy-l'Etoile, 69280, France.
Université de Lyon, École Centrale de Lyon, Ecully, 69134, France.
BMC Musculoskelet Disord. 2024 Dec 19;25(1):1018. doi: 10.1186/s12891-024-07921-0.
This study aimed to assess the effects of an antioxidant-conjugated Hyaluronic Acid (HA), specifically HA-4-aminoresorcinol (HA4AR), on articular cartilage and subchondral bone in osteoarthritis (OA). We conducted a comparative analysis between HA4AR and a commercially available high molecular weight HA formulation in a rabbit model of OA.
Eighteen rabbits underwent unilateral anterior cruciate ligament transection (ACLT) and were divided into three groups of six: Saline-group, HA-group, and HA4AR-group, based on the type of intra-articular injection received. Additionally, eight non-operated contralateral knees served as reference points (Contralateral-group). Six weeks post-surgery, iodine-enhanced micro-computed tomography imaging was used to evaluate articular cartilage volume and thickness, and to assess subchondral bone microarchitecture and mineral density.
Cartilage thickness in both the HA and HA4AR groups was comparable to that of the Contralateral group. Notably, there was a significant reduction in subchondral bone plate tissue mineral density in the HA-group when compared to both the HA4AR and Saline groups (p < 0.05). However, no significant differences in trabecular subchondral bone microarchitectural parameters and mineral densities were observed between the HA4AR-group and the Saline-group. When compared to the Contralateral, Saline, and HA4AR groups, the HA-group exhibited a marked decrease in subchondral bone plate tissue mineral density (p < 0.05). Additionally, a significant reduction in trabecular bone volume fraction was noted in the HA-group compared to the Contralateral-group (p < 0.05).
The HA-4AR hydrogel demonstrated significant preservation of subchondral bone plate tissue mineral density compared to HA alone, while other bone microarchitectural parameters remained largely unchanged. These findings indicate that HA-4AR may provide enhanced osteoprotective benefits in the treatment of osteoarthritis.
本研究旨在评估抗氧化剂共轭透明质酸（HA），特别是HA - 4 - 氨基间苯二酚（HA4AR）对骨关节炎（OA）关节软骨和软骨下骨的影响。我们在兔OA模型中对HA4AR和市售高分子量HA制剂进行了比较分析。
18只兔子接受单侧前交叉韧带切断术（ACLT），并根据关节内注射类型分为三组，每组6只：生理盐水组、HA组和HA4AR组。另外，8只未手术的对侧膝关节作为对照点（对侧组）。术后6周，使用碘增强微型计算机断层扫描成像评估关节软骨体积和厚度，并评估软骨下骨微结构和矿物质密度。
HA组和HA4AR组的软骨厚度与对侧组相当。值得注意的是，与HA4AR组和生理盐水组相比，HA组软骨下骨板组织矿物质密度显著降低（p < 0.05）。然而，HA4AR组和生理盐水组之间在小梁软骨下骨微结构参数和矿物质密度方面未观察到显著差异。与对侧组、生理盐水组和HA4AR组相比，HA组软骨下骨板组织矿物质密度显著降低（p < 0.05）。此外，与对侧组相比，HA组小梁骨体积分数显著降低（p < 0.05）。
与单独使用HA相比，HA - 4AR水凝胶在软骨下骨板组织矿物质密度的保存方面表现出显著效果，而其他骨微结构参数基本保持不变。这些发现表明，HA - 4AR在骨关节炎治疗中可能提供增强的骨保护益处。
