Alric Hadrien, Mathieu Noëlle, Sebbagh Anna, Peré Guillaume, Demarquay Christelle, Cronemberger André, Berger Arthur, Marcel Benjamin, Wilhelm Claire, Gazeau Florence, Mariani Antoine, Karoui Mehdi, Clément Olivier, Araujo-Filho Irami, Silva Amanda K A, Rahmi Gabriel
Laboratoire de Recherche en Imagerie du Vivant, PARCC, INSERM U970, Université Paris Cité, Paris, France.
Service d'Hépato-Gastro-Entérologie et Endoscopies Digestives, Hôpital Européen Georges Pompidou, APHP.Centre-Université Paris Cité, Paris, France.
Commun Biol. 2024 Dec 19;7(1):1673. doi: 10.1038/s42003-024-07364-2.
Anastomotic leak occurrence is a severe complication after colorectal surgery. Considering the difficulty of treating these leaks and their impact on patient care, there is a strong need for an efficient prevention strategy. We evaluated a combination of extracellular vesicles (EVs) from rat adipose-derived stromal cells with a thermoresponsive gel, Pluronic® F127 (PF-127) to prevent anastomotic leaks. The pro-regenerative and immunomodulatory potencies of EVs are assessed in vitro. In vivo efficacy are assessed in rat with a colonic anastomosis model after irradiation. Endoscopic, anatomical and histological data show a consistent effect of EVs + gel on the healing of colonic anastomosis. These results are illustrated by a smaller anastomotic ulcer size, less fibrosis and less inflammatory infiltrations in the EVs + gel group. This multi-modal investigation is the first to point-out the translational potential of EVs combined with PF-127 for the healing of high-risk colorectal anastomosis.
吻合口漏是结直肠手术后的一种严重并发症。考虑到治疗这些漏口的难度及其对患者护理的影响，迫切需要一种有效的预防策略。我们评估了来自大鼠脂肪来源基质细胞的细胞外囊泡（EVs）与热敏凝胶泊洛沙姆F127（PF-127）联合使用以预防吻合口漏。在体外评估了EVs的促再生和免疫调节能力。在辐射后的大鼠结肠吻合模型中评估体内疗效。内镜、解剖学和组织学数据显示，EVs+凝胶对结肠吻合口愈合具有一致的效果。EVs+凝胶组吻合口溃疡尺寸更小、纤维化程度更低、炎症浸润更少，这些结果证明了上述效果。这项多模式研究首次指出了EVs与PF-127联合用于高危结直肠吻合口愈合的转化潜力。
