He Chunlin, Zhang Xinhui, Wen Zhengyong, Shi Qiong, Song Zhaobin
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518057, China.
Sci Data. 2024 Dec 19;11(1):1404. doi: 10.1038/s41597-024-04268-y.
In this study, we constructed a chromosome-scale reference genome assembly for Lixian plateau loach, Triplophysa lixianensis, by integration of MGI short-read, PacBio HiFi long-read and Hi-C sequencing technologies. A 668-Mb haplotypic genome assembly was obtained for a female T. lixianensis, and 98.91% of the assembled sequences were anchored into 25 chromosomes. This assembly owned a moderate repeat content (35.63%) and an annotation of 23,774 protein-coding genes, among them 94.15% were predicted with functions. The assembled genome of T. lixianensis shared a good syntenic relationship with previously published data of its relative T. dalaica. Taken together, our genome data presented here provide a valuable genetic resource for in-depth evolutionary and functional studies, as well as molecular breeding and conservation of this valuable fish species to elevate its ecological and economical values.
在本研究中，我们通过整合MGI短读长、PacBio HiFi长读长和Hi-C测序技术，构建了理县高原鳅（Triplophysa lixianensis）的染色体水平参考基因组组装。获得了一个雌性理县高原鳅的668 Mb单倍型基因组组装，其中98.91%的组装序列被锚定到25条染色体上。该组装具有适度的重复序列含量（35.63%），并注释了23774个蛋白质编码基因，其中94.15%的基因具有预测功能。理县高原鳅的组装基因组与其近缘物种达里湖高原鳅（T. dalaica）先前发表的数据具有良好的共线性关系。综上所述，我们在此展示的基因组数据为深入的进化和功能研究以及该珍贵鱼类物种的分子育种和保护提供了宝贵的遗传资源，以提升其生态和经济价值。
