Fisheries Research Institute of Fujian, Xiamen, 361000, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
Sci Data. 2023 Jan 11;10(1):22. doi: 10.1038/s41597-023-01937-2.
Takifugu species serve as a model system for evolutionary studies due to their compact genomes and diverse phenotypes. The ocellated puffer (Takifugu ocellatus), characterized by special colouration, is a scarce anadromous species in the genus Takifugu. As an ornamental and tasty fish species, T. ocellatus has moderate economic value. However, the available genomic resources for this pufferfish are still limited. Here, a chromosome-level reference genome, as well as two haploid genomes, was constructed by PacBio HiFi long sequencing and Hi-C technologies. The total length of the reference genome was 375.62 Mb with a contig N50 of 11.55 Mb. The assembled sequences were anchored to 22 chromosomes with an integration efficiency of 93.78%. Furthermore, 28,808 protein-coding genes were predicted. The haplotype-resolved reference genome of T. ocellatus provides a crucial resource for investigating the explosive speciation of the Takifugu genus, such as elucidating evolutionary histories, determining the genetic basis of trait evolution, and supporting future conservation efforts.
由于其紧凑的基因组和多样的表型，河豚物种是进化研究的模型系统。眼斑东方鲀（Takifugu ocellatus）是东方鲀属中一种具有特殊颜色的稀有洄游物种。作为一种观赏和美味的鱼类，眼斑东方鲀具有中等的经济价值。然而，这种河豚鱼的可用基因组资源仍然有限。在这里，通过 PacBio HiFi 长测序和 Hi-C 技术构建了一个染色体水平的参考基因组和两个单倍体基因组。参考基因组的总长度为 375.62Mb，其拼接 contig N50 为 11.55Mb。组装的序列被锚定到 22 条染色体上，整合效率为 93.78%。此外，预测了 28808 个蛋白质编码基因。眼斑东方鲀的单倍型解析参考基因组为研究东方鲀属的爆发式物种形成提供了重要资源，例如阐明进化历史、确定性状进化的遗传基础，以及支持未来的保护工作。
