Shu Fuxing, Sarsaiya Surendra, Ren Lili, Jin Leilei, Hu Yuhe, Qiao Ling, Xu Xiaoqing, Chen Guoguang, Chen Jishuang
School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu, China.
Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, Guizhou, China.
Physiol Plant. 2024 Nov-Dec;176(6):e70016. doi: 10.1111/ppl.70016.
Plant-derived nanovesicles (PDNVs) and extracellular vesicles (EVs) represent a promising area of research due to their unique properties and potential therapeutic applications. Pinellia ternata (P. ternata) is well-known for its pharmacological properties but the PDNVs and EVs derived from it have been largely understudied. Previous studies have shown that a Temporary Immersion Bioreactor System (TIBS) plays an important role in controlling plant growth in order to obtain reproducible EVs and PDNVs. PDNVs were isolated from P. ternata plants and EVs were collected in the TIBS medium via ultra-high-speed differential centrifugation. Particle size, Zeta potentials and particle concentrations were assessed for PDNVs and EVs. Furthermore, non-targeted metabolomics was used to assess metabolic compositional differences between EVs and PDNVs, enabling the evaluation of the TIBS's quality control efficacy. Metabolomic profiling revealed 1072 metabolites in PDNVs and EVs, including 426 differential metabolites (DMs) distinguishing PDNVs from EVs: 362 DMs were positively correlated with PDNVs and 64 DMs were positively correlated with EVs; they were enriched across 17 KEGG pathways. PCA, PLS-DA, and metabolite sample correlation analyses showed high consistency between the replicates (PDNVs >0.87, EVs >0.93). This study demonstrated that TIBS is a performant system allowing consistency in generating PDNVs and EVs from P. ternata. We also highlighted the metabolic differences between PDNVs and EVs, guiding researchers in finding the bet system to produce efficient nanodrugs containing P. ternata pharmacological compounds.
植物源纳米囊泡（PDNVs）和细胞外囊泡（EVs）因其独特的性质和潜在的治疗应用而成为一个有前景的研究领域。半夏以其药理特性而闻名，但源自半夏的PDNVs和EVs在很大程度上尚未得到充分研究。先前的研究表明，临时浸没生物反应器系统（TIBS）在控制植物生长以获得可重复的EVs和PDNVs方面发挥着重要作用。从半夏植株中分离出PDNVs，并通过超高速差速离心法在TIBS培养基中收集EVs。对PDNVs和EVs的粒径、zeta电位和颗粒浓度进行了评估。此外，非靶向代谢组学被用于评估EVs和PDNVs之间的代谢成分差异，从而能够评估TIBS的质量控制效果。代谢组学分析揭示了PDNVs和EVs中的1072种代谢物，包括426种区分PDNVs和EVs的差异代谢物（DMs）：362种DMs与PDNVs呈正相关，64种DMs与EVs呈正相关；它们在17条KEGG途径中富集。主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和代谢物样本相关性分析表明，各重复样本之间具有高度一致性（PDNVs>0.87，EVs>0.93）。本研究表明，TIBS是一个性能良好的系统，能够一致地从半夏中生成PDNVs和EVs。我们还强调了PDNVs和EVs之间的代谢差异，为研究人员寻找生产含有半夏药理化合物的高效纳米药物的最佳系统提供了指导。
