Pharmacy Faculty, Hubei University of Chinese Medicine, Wuhan 430065, China; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Pharmacy Faculty, Hubei University of Chinese Medicine, Wuhan 430065, China.
J Environ Manage. 2024 Dec;371:123322. doi: 10.1016/j.jenvman.2024.123322. Epub 2024 Nov 15.
Recent research has reported the strong herbicidal activity of Artemisia argyi leaf powder (AALP), indicating its high potential for use as an environmentally friendly weed management solution for ecological agriculture. However, AALP's impacts on soil physicochemical properties and microbial communities have remained uninvestigated. This study explores these effects through pot experiments assessing the AALP's efficacy in weed suppression and its ability to promote the growth of Pinellia ternata, a plant utilized in traditional Chinese medicine. The results demonstrate that a 10% concentration of AALP suppressed nearly 100% of all weeds. Additionally, AALP treatments at 2.5%, 5%, 7.5%, and 10% concentrations increased P. ternata yields by 29.79%, 24.76%, 35.67%, and 31.00%, respectively. A soil analysis revealed that AALP enhanced soil fertility by increasing the contents of nutrients such as SOM, AN, AP, AK, Ca, Fe, Mn, and Zn, as well as the enzyme activity of CAT, ACP, UE, and SC, creating an optimal growth environment for P. ternata. In addition, AALP significantly increased the PA (phenolic acid) content in soil, which is a key factor in inhibiting weed germination and growth. Furthermore, a microbial community structure analysis indicated an enrichment of Actinobacteriota and Bacteroidota after AALP treatment, with notable increases in the growth-promoting bacteria Sphingobium and Flavobacterium. A permutational multivariate analysis of variance (PERMANOVA) based on the Bray-Curtis distance reaveled that all of the tested soil properties were significantly correlated with changes in bacterial community composition except for pH. Further two-factor correlation network analysis identified AN, Zn, SC, and PA as key environmental factors. Finally, the Sphingobium sp. strain AFR15, isolated from AALP-treated soil, exhibited significant growth-promoting effects on P. ternata. After inoculation with Sphingobium sp. strain AFR15 for one month, the heights of P. ternata were increased significantly. The leaf length and leaf width of P. ternata were also positively correlated with the treatment concentration of AFR15, and the chlorophyll contents of the leaves also increased. This results highlighted Sphingobium sp. strain AFR15's potential as a specialized microbial fertilizer in crop yield increased. In conclusion, AALP applications not only control weeds but also promote P. ternata growth by improving soil physiochemical properties and fostering beneficial bacterial allies. These findings lay the groundwork for future research and promote the use of AALP in ecological agriculture.
最近的研究报告称，艾叶粉（AALP）具有很强的除草活性，这表明它有很大的潜力可用作生态农业中环保的杂草管理解决方案。然而，AALP 对土壤理化性质和微生物群落的影响仍未得到研究。本研究通过盆栽实验探讨了这些效应，评估了 AALP 在抑制杂草方面的功效以及促进中药半夏生长的能力。结果表明，10%浓度的 AALP 抑制了近 100%的所有杂草。此外，AALP 处理浓度为 2.5%、5%、7.5%和 10%时，半夏的产量分别增加了 29.79%、24.76%、35.67%和 31.00%。土壤分析表明，AALP 通过增加 SOM、AN、AP、AK、Ca、Fe、Mn 和 Zn 等养分以及 CAT、ACP、UE 和 SC 等酶的活性，提高了土壤肥力，为半夏创造了最佳的生长环境。此外，AALP 显著增加了土壤中酚酸（PA）的含量，这是抑制杂草发芽和生长的关键因素。此外，微生物群落结构分析表明，AALP 处理后 Actinobacteriota 和 Bacteroidota 富集，生长促进菌 Sphingobium 和 Flavobacterium 显著增加。基于 Bray-Curtis 距离的可置换多元方差分析（PERMANOVA）表明，除 pH 外，所有测试的土壤性质均与细菌群落组成的变化显著相关。进一步的双因素相关网络分析确定了 AN、Zn、SC 和 PA 是关键的环境因素。最后，从 AALP 处理过的土壤中分离到的 Sphingobium sp. 菌株 AFR15 对半夏表现出显著的促生作用。用 Sphingobium sp. 菌株 AFR15 接种一个月后，半夏的高度显著增加。半夏的叶片长度和叶片宽度也与 AFR15 的处理浓度呈正相关，叶片中的叶绿素含量也增加。这一结果突显了 Sphingobium sp. 菌株 AFR15 作为一种专门的微生物肥料在提高作物产量方面的潜力。总之，AALP 的应用不仅可以控制杂草，还可以通过改善土壤理化性质和培育有益的细菌伙伴来促进半夏的生长。这些发现为未来的研究奠定了基础，并促进了 AALP 在生态农业中的应用。
