Cerruti Paolo, Campobenedetto Cristina, Montrucchio Elisa, Agliassa Chiara, Contartese Valeria, Acquadro Alberto, Bertea Cinzia Margherita
Department of Agricultural, Forest and Food Sciences, University of Turin, Grugliasco, Italy.
Green Has Italia, Canale, Italy.
Physiol Plant. 2024 Nov-Dec;176(6):e70007. doi: 10.1111/ppl.70007.
Drought is a significant global environmental stress. Biostimulants offer a sustainable solution to enhance crop tolerance and mitigate productivity losses. This study assessed the impact of foliar application of ERANTHIS®, a biostimulant derived from the algae Ascophyllum nodosum and Laminaria digitata and yeast extracts, on tomato plants under mild water stress. Evaluations were conducted at 5 and 24 hours after the third treatment. Under optimal water conditions, the biostimulant showed a priming effect, with an early increase of stress markers and a timing-specific modulation of ROS non enzymatic and enzymatic ROS scavenging activities. Under drought stress, the biostimulant later decreased stress markers, by aligning the majority of analyzed ROS scavengers closer to levels in well-irrigated plants. Transcriptome analysis using RNA-seq data revealed differentially expressed genes (DEGs) and multivariate data highlighted groups of co-regulated genes (k-means clustering). Genes involved in water channel activity, transcription regulator activity, and oxidoreductase activity were significantly modulated. Cluster analysis identified distinct gene clusters influenced by the biostimulant under optimal conditions, including early responses (cell wall modification, hormone signaling) and late responses (RNA modification, nutrient uptake process). Under water stress, early responses involved actin filament organization and MAPK signaling, while late responses were related to plasma membrane components and cell wall organization. This study, integrating biochemical and transcriptomic data, provides a comprehensive understanding of how a biostimulant primes plants under optimal conditions and mitigates water stress effects, offering valuable insights for sustainable agriculture.
干旱是一种严重的全球环境压力。生物刺激素提供了一种可持续的解决方案，以提高作物耐受性并减轻生产力损失。本研究评估了叶面喷施ERANTHIS®（一种源自海带和掌状海带以及酵母提取物的生物刺激素）对轻度水分胁迫下番茄植株的影响。在第三次处理后的5小时和24小时进行了评估。在最佳水分条件下，生物刺激素显示出一种引发效应，应激标志物早期增加，活性氧非酶促和酶促清除活性呈现出特定时间的调节。在干旱胁迫下，生物刺激素随后降低了应激标志物，使大多数分析的活性氧清除剂更接近充分灌溉植株的水平。使用RNA测序数据进行的转录组分析揭示了差异表达基因（DEGs），多变量数据突出了共调控基因组（k均值聚类）。参与水通道活性、转录调节活性和氧化还原酶活性的基因受到显著调节。聚类分析确定了在最佳条件下受生物刺激素影响的不同基因簇，包括早期反应（细胞壁修饰、激素信号传导）和晚期反应（RNA修饰、养分吸收过程）。在水分胁迫下，早期反应涉及肌动蛋白丝组织和丝裂原活化蛋白激酶信号传导，而晚期反应与质膜成分和细胞壁组织有关。本研究整合了生化和转录组数据，全面了解了生物刺激素如何在最佳条件下引发植物并减轻水分胁迫效应，为可持续农业提供了有价值的见解。
