Tugui Claudia G, Sorokin Dimitry Y, Hijnen Wim, Wunderer Julia, Bout Kaatje, van Loosdrecht Mark C M, Pabst Martin
Delft University of Technology, Department of Biotechnology Delft The Netherlands
Winogradsky Institute of Microbiology, Federal Research Centre of Biotechnology, RAS Moscow Russia.
RSC Chem Biol. 2024 Dec 9;6(2):227-239. doi: 10.1039/d4cb00200h. eCollection 2025 Feb 5.
Members of the genus are commonly found in natural aquatic ecosystems. However, they are also frequently present in non-chlorinated drinking water distribution systems. High densities of these bacteria indicate favorable conditions for microbial regrowth, which is considered undesirable. Studies have indicated that the presence of is associated with loose deposits and the presence of invertebrates, specifically . Therefore, a potential source of energy in these nutrient poor environments is chitin, the structural shell component in these invertebrates. In this study, we demonstrate the ability of two strains, commonly encountered in drinking water distribution systems, to effectively degrade and utilize chitin as a sole carbon and nitrogen source. We conducted a quantitative proteomics study on the cell biomass and secretome from pure strain cultures when switching the nutrient source from glucose to chitin, uncovering a diverse array of hydrolytic enzymes and metabolic pathways specifically dedicated to the utilization of chitin. Additionally, a genomic analysis of different species suggests the general ability of this genus to degrade and utilize a variety of carbohydrate biopolymers. This study indicates the relation between the utilization of chitin by and their association with invertebrates such as in loose deposits in drinking water distribution systems.
该属成员常见于天然水生生态系统中。然而，它们也经常出现在未氯化的饮用水分配系统中。这些细菌的高密度表明有利于微生物再生长的条件，而这被认为是不理想的。研究表明，[具体细菌名称]的存在与松散沉积物以及无脊椎动物的存在有关，特别是[具体无脊椎动物名称]。因此，在这些营养贫乏的环境中，一个潜在的能量来源是几丁质，即这些无脊椎动物的结构外壳成分。在本研究中，我们证明了在饮用水分配系统中常见的两种[具体细菌名称]菌株能够有效地降解并利用几丁质作为唯一的碳源和氮源。当将营养源从葡萄糖切换到几丁质时，我们对纯菌株培养物的细胞生物质和分泌组进行了定量蛋白质组学研究，发现了一系列专门用于利用几丁质的水解酶和代谢途径。此外，对不同[具体细菌名称]物种的基因组分析表明该属具有降解和利用多种碳水化合物生物聚合物的一般能力。这项研究表明了[具体细菌名称]对几丁质的利用与其在饮用水分配系统的松散沉积物中与[具体无脊椎动物名称]等无脊椎动物的关联之间的关系。
