Islam Hashim, Boultbee Jordan, Jackson Garett S, Mui Alice L, Little Jonathan P
School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, BC, Canada.
Centre for Chronic Disease Prevention and Management, University of British Columbia Okanagan, Kelowna, BC, Canada.
Transl Exerc Biomed. 2024 Nov 14;1(3-4):255-263. doi: 10.1515/teb-2024-0027. eCollection 2024 Sep.
To determine how the anti-inflammatory actions of interleukin-10 (IL-10) and IL-6 differ across age and physical activity levels.
Using a cross-sectional design, fasted blood samples were obtained from younger physically inactive (YI: n=10, age: 22.7 ± 3.7 years, BMI: 24.8 ± 4.8 kg/m, <150 min of weekly moderate-to-vigorous physical activity [MVPA]), younger highly active (YA: n=11 varsity cross country running athletes, 20.7 ± 2.7 years, 21.1 ± 1.8 kg/m, >300 min of weekly MVPA), and older highly active (OA: 12, 56.0 ± 10.3 years, 22.8 ± 3.2 kg/m, >300 min of weekly MVPA) individuals and analyzed for leukocyte counts, IL-10 and IL-6-related signaling, and cytokine secretion
Total white blood cells and monocytes were similar between groups (p=0.8) but YA and OA had lower lymphocyte counts than YI (p<0.01). The ability of IL-10 (1 ng/mL) to phosphorylate signal transducer and activator of transcription 3 (STAT3) in CD14 monocytes was greater in YA vs. YI (p<0.03) despite YA having lower IL-10 receptor expression (p<0.01). IL-6 (10 ng/mL) mediated STAT3 phosphorylation in CD4 lymphocytes was higher in OA compared YI (p<0.01), with a similar tendency observed for YA vs. YI (p=0.08). Despite enhanced responsiveness of STAT3 to IL-10/6 in active individuals, the ability of IL-10/6 to inhibit tumor necrosis factor-alpha (TNF-⍺) secretion from lipopolysaccharide-stimulated whole-blood was similar between groups.
Highly active younger and older individuals demonstrate enhanced IL-10- and IL-6-mediated activation of immune cell STAT3. Although the ability of IL-10/6 to inhibit TNF-⍺ secretion appeared unimpacted by activity level, anti-inflammatory cytokine actions were preserved in older active individuals.
确定白细胞介素-10（IL-10）和IL-6的抗炎作用在不同年龄和身体活动水平之间有何差异。
采用横断面设计，从以下人群中获取空腹血样：年轻的缺乏身体活动者（YI：n = 10，年龄：22.7±3.7岁，体重指数：24.8±4.8kg/m²，每周中度至剧烈身体活动[MVPA]少于150分钟）、年轻的高活动量者（YA：n = 11名大学越野跑运动员，20.7±2.7岁，21.1±1.8kg/m²，每周MVPA超过300分钟）以及年长的高活动量者（OA：12名，56.0±10.3岁，22.8±3.2kg/m²，每周MVPA超过300分钟），并分析白细胞计数、IL-10和IL-6相关信号传导以及细胞因子分泌情况。
各组之间的总白细胞和单核细胞数量相似（p = 0.8），但YA组和OA组的淋巴细胞计数低于YI组（p<0.01）。尽管YA组的IL-10受体表达较低（p<0.01），但IL-10（1ng/mL）使CD14单核细胞中的信号转导和转录激活因子3（STAT3）磷酸化的能力在YA组中比YI组更强（p<0.03）。与YI组相比，OA组中IL-6（10ng/mL）介导的CD4淋巴细胞中STAT3磷酸化水平更高（p<0.01），YA组与YI组相比也有类似趋势（p = 0.08）。尽管活跃个体中STAT3对IL-10/6的反应性增强，但各组之间IL-10/6抑制脂多糖刺激全血中肿瘤坏死因子-α（TNF-α）分泌的能力相似。
高活动量的年轻和年长个体表现出IL-10和IL-6介导的免疫细胞STAT3激活增强。尽管IL-10/6抑制TNF-α分泌的能力似乎不受活动水平影响，但年长的活跃个体中抗炎细胞因子的作用得以保留。
