Gürgen Ayşenur, Unal Orhan, Sevindik Mustafa
University of Osmaniye Korkut Ata, Faculty of Engineering and Nature Sciences, Department of Industrial Engineering, Osmaniye, Turkey.
University of Akdeniz, Faculty of Science, Deparment of Biology, Antalya, Turkey.
Int J Med Mushrooms. 2024;26(12):63-74. doi: 10.1615/IntJMedMushrooms.2024055569.
In our study, the biological activities of the wild edible mushroom Cantharellus cibarius were determined. First of all, 64 different experiments were performed in the Soxhlet device at 40-70°C extraction temperatures, 4, 6, 8, and 10 h of extraction time and 0.25, 0.50, 1, and 2 mg/mL extract concentrations, and total antioxidant (TAS) and total oxidant values (TOS) were determined, and both single-purpose and multi-objective optimization studies were performed. The extraction conditions were optimized so that the objective function of the single-purpose optimization would be the maximum TAS value, and the objective function of the multi-objective optimization would be the maximum TAS and minimum TOS values. The data obtained from the experimental study were modeled with artificial neural networks from artificial intelligence methods, and optimization was performed with a genetic algorithm. After the single-objective optimization process, the optimum extraction conditions were found as 46.622°C, 5.110 h and 1.973 mg/mL and the multi-objective optimum extraction conditions were found as 50.927°C, 6.094 h and 1.439 mg/mL. The TAS values of the extracts obtained as a result of single-objective and multi-objective optimization were measured as 5.511 and 5.359, respectively, and the TOS values were measured as 7.289 and 6.061. The anti-AChE value of the extracts was calculated as 16.12, 20.31 and anti-BChE value as 36.92, 40.28. In addition, it was determined that the single-objective optimization extract had stronger antiproliferative activity than the multi-objective extract. As a result, it was determined that the biological activities of the extracts of C. cibarius obtained under suitable conditions were high.
在我们的研究中，测定了野生可食用蘑菇鸡油菌的生物活性。首先，在索氏提取器中于40 - 70°C的提取温度、4、6、8和10小时的提取时间以及0.25、0.50、1和2 mg/mL的提取物浓度下进行了64次不同的实验，测定了总抗氧化能力（TAS）和总氧化值（TOS），并进行了单目标和多目标优化研究。优化提取条件，使单目标优化的目标函数为最大TAS值，多目标优化的目标函数为最大TAS值和最小TOS值。将实验研究获得的数据用人工智能方法中的人工神经网络进行建模，并用遗传算法进行优化。单目标优化过程后，发现最佳提取条件为46.622°C、5.110小时和1.973 mg/mL，多目标最佳提取条件为50.927°C、6.094小时和1.439 mg/mL。单目标和多目标优化得到的提取物的TAS值分别测定为5.511和5.359，TOS值分别测定为7.289和6.061。提取物的抗乙酰胆碱酯酶（AChE）值计算为16.12、20.31，抗丁酰胆碱酯酶（BChE）值计算为36.92、40.28。此外，确定单目标优化提取物比多目标提取物具有更强的抗增殖活性。结果表明，在合适条件下获得的鸡油菌提取物的生物活性较高。
