Lundquist Patrik, Hagforsen Eva, Wagner Michael, Alimohammadi Mohammad, Melo Fabio Rabelo, Pejler Gunnar, Artursson Per, Carlson Marie, Rollman Ola, Lampinen Maria
Department of Pharmacy, Uppsala University, Box 580, SE-751 23 Uppsala, Sweden.
Dermatology and Venereology, Department of Medical Sciences, Uppsala University Hospital, SE-751 85 Uppsala, Sweden.
Biochim Biophys Acta Mol Basis Dis. 2025 Mar;1871(3):167634. doi: 10.1016/j.bbadis.2024.167634. Epub 2024 Dec 19.
Psoriasis is a chronic skin disease occasionally associated with abdominal symptoms and IBD. We aimed to characterize intestinal immune cells and the integrity of the intestinal barrier in psoriasis. Biopsies from the duodenum and colon were analyzed by flow cytometry and immunohistochemistry for the presence and activation status of different immune cell populations. Intestinal permeability was measured using Ussing chambers. Proinflammatory markers were analyzed in fecal and blood samples using ELISA. The intestinal level of inflammatory mediators was assessed using a multiplex proximity extension assay. We found an increased density of intestinal eosinophils, mast cells, macrophages, and CD8 T-cells in psoriasis; eosinophils, macrophages, and CD8+ T-cells expressed activation markers. Half of the psoriasis patients showed increased permeability across the duodenum, correlating with increased mucosal IL-17A, IL-13, IL-2, and IL-20, and with gastrointestinal symptoms. Our findings reveal that psoriasis is associated with low-grade intestinal inflammation, which may contribute to abdominal symptoms in these patients and possibly set the stage for the development of intestinal disease.
银屑病是一种慢性皮肤病，偶尔与腹部症状和炎症性肠病相关。我们旨在描述银屑病患者肠道免疫细胞及肠道屏障的完整性。通过流式细胞术和免疫组织化学分析十二指肠和结肠活检样本中不同免疫细胞群的存在情况和激活状态。使用尤斯灌流小室测量肠道通透性。采用酶联免疫吸附测定法分析粪便和血液样本中的促炎标志物。使用多重邻近延伸分析评估肠道炎症介质水平。我们发现银屑病患者肠道嗜酸性粒细胞、肥大细胞、巨噬细胞和CD8 T细胞密度增加；嗜酸性粒细胞、巨噬细胞和CD8 + T细胞表达激活标志物。半数银屑病患者十二指肠通透性增加，这与黏膜白细胞介素-17A、白细胞介素-13、白细胞介素-2和白细胞介素-20水平升高以及胃肠道症状相关。我们的研究结果表明，银屑病与低度肠道炎症相关，这可能导致这些患者出现腹部症状，并可能为肠道疾病的发展奠定基础。
