Ohtawara M, Kobune F, Umino Y, Sugiura A
Arch Virol. 1985;83(3-4):217-27. doi: 10.1007/BF01309918.
We have compared the kinetic growth patterns of To-336, MEQ11, KRT, and SK2 rubella vaccine strains licensed in Japan at 37 degrees and 39 degrees C with those of progenitor wild strains of rubella virus. The growth of vaccine strains was depressed at 39 degrees C to a level about 3 log10 lower than that at 37 degrees C. The difference in virus titer attained by wild strains at 37 degrees and 39 degrees C was less than tenfold. The growth potential at 39 degrees C paralleled the immunogenic marker of rubella virus, i.e. the capability of virus to induce antibody response upon subcutaneous injection in rabbits for all wild and vaccine strains examined, including one strain at an intermediate level of attenuation. Several clones were isolated from the progeny produced by a vaccine strain during the growth at 39 degrees C. Among them were partial revertants in immunogenic marker as well as in the growth potential at 39 degrees C. It was concluded that the immunogenic marker of rubella virus in rabbits represented its capability to replicate at the body temperature of the animal.
我们比较了日本许可的To-336、MEQ11、KRT和SK2风疹疫苗株在37℃和39℃下的动力学生长模式与风疹病毒原始野生株的生长模式。疫苗株在39℃下的生长受到抑制，其水平比在37℃下低约3个对数10。野生株在37℃和39℃下达到的病毒滴度差异小于10倍。39℃下的生长潜力与风疹病毒的免疫原性标志物平行，即对于所有检测的野生株和疫苗株，包括一株处于中等减毒水平的毒株，病毒在兔皮下注射后诱导抗体反应的能力。在39℃生长期间，从疫苗株产生的子代中分离出几个克隆。其中有免疫原性标志物以及39℃下生长潜力的部分回复株。得出的结论是，风疹病毒在兔中的免疫原性标志物代表了其在动物体温下复制的能力。
