Lv Xiaoqian, Wang Xiaoyu, Yue Jianxing, Wang Xin, Chen Haoyue, Gao Qiang
The Second Affiliated Hospital of Bengbu Medical University, Bengbu, 233040, China.
The Affiliated Taian City Central Hospital of Qingdao University, Taian, 271000, China.
Stem Cell Res Ther. 2024 Dec 20;15(1):490. doi: 10.1186/s13287-024-04108-1.
In the rat knee stiffness model, the duration of traction treatment is mostly 20-40 min; however, relatively few studies have been conducted on longer traction treatment of extended knee stiffness in rats. Therefore, the aim of this study was to explore the efficacy of prolonged traction and its mechanism of action in extended knee stiffness in rats.
The model of extended knee joint stiffness was established in rats and treated with powered flexion position traction. On the 10th and 20th days respectively, passive range of motion (PROM) assessments and musculoskeletal ultrasound were conducted. Rectus femoris muscle tissues were taken for Western blotting (WB) to detect the expression of muscle satellite cells proliferation and differentiation signaling factors. Histopathological staining was used to evaluate the degree of muscle atrophy and muscle fibrosis in the rectus femoris muscle, and immunofluorescence double staining was used to detect proliferation of muscle satellite cells number. The results from these analyses were used to assess the therapeutic outcomes of the traction treatment.
The findings indicated that chronic persistent traction significantly improved joint mobility, notably enhanced the proliferation of muscle satellite cells, and inhibited their differentiation. Furthermore, the treatment facilitated the repair and regeneration of damaged tissues, reduced muscular atrophy and fibrosis in the rectus femoris muscle, and alleviated knee stiffness.
Chronic persistent traction can effectively relieve knee joint stiffness, and its mechanism is related to the activation and proliferation of the rectus femoris muscle satellite cells, thereby promoting the repair and regeneration of damaged skeletal muscle.
在大鼠膝关节僵硬模型中，牵引治疗的持续时间大多为20 - 40分钟；然而，针对大鼠膝关节长期僵硬进行更长时间牵引治疗的研究相对较少。因此，本研究的目的是探讨延长牵引对大鼠膝关节僵硬的疗效及其作用机制。
建立大鼠膝关节伸展僵硬模型，并采用动力屈曲位牵引进行治疗。分别在第10天和第20天进行被动活动范围（PROM）评估和肌肉骨骼超声检查。取股直肌组织进行蛋白质免疫印迹法（WB）检测，以检测肌肉卫星细胞增殖和分化信号因子的表达。采用组织病理学染色评估股直肌的肌肉萎缩和肌肉纤维化程度，采用免疫荧光双染色检测肌肉卫星细胞的增殖数量。这些分析结果用于评估牵引治疗的疗效。
研究结果表明，慢性持续牵引可显著改善关节活动度，显著增强肌肉卫星细胞的增殖，并抑制其分化。此外，该治疗促进了受损组织的修复和再生，减轻了股直肌的肌肉萎缩和纤维化，缓解了膝关节僵硬。
慢性持续牵引可有效缓解膝关节僵硬，其机制与股直肌卫星细胞的激活和增殖有关，从而促进受损骨骼肌的修复和再生。
