Sun Luying, Jia Minyi, Zhu Kui, Hao Zhihui, Shen Jianzhong, Wang Shaolin
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Beijing, People's Republic of China.
Probiotics Antimicrob Proteins. 2024 Dec 21. doi: 10.1007/s12602-024-10424-y.
The escalating prevalence of antibiotic-resistant bacteria has emerged as a formidable threat to global health, and the quest for alternative antimicrobial agents is imperative. Cecropins, a class of antimicrobial peptides (AMPs), have garnered attention due to their potent bactericidal properties. This investigation delves into the antibacterial prowess of Cecropin A (CA) and Cecropin AD (CAD), showcasing their robust activity against Gram-negative bacteria, inclusive of multidrug-resistant bacteria. The bactericidal efficacy of CA and CAD is characterized by a dose-responsive paradigm, affirming their potential as therapeutic agents. These peptides exhibit minimal cytotoxicity and hemolytic effects, underscoring their safety profile. Advanced experimentation has elucidated that cecropins could disrupt the outer bacterial membrane, targeting lipid A, a pivotal constituent of the lipopolysaccharides (LPS) in the outer membrane as their antimicrobial bullseye. The affinity of cecropins for LPS and their antimicrobial action underscore the therapeutic potential of these peptides in targeting Gram-negative bacterial infections. These insights accentuate the promise of cecropins as viable "antibiotic substitutes," paving the path for their expanded application in combating antibiotic resistance.
抗生素耐药细菌的日益流行已成为对全球健康的巨大威胁，因此寻求替代抗菌剂势在必行。天蚕素是一类抗菌肽，因其强大的杀菌特性而受到关注。本研究深入探讨了天蚕素A（CA）和天蚕素AD（CAD）的抗菌能力，展示了它们对革兰氏阴性菌（包括多重耐药菌）的强大活性。CA和CAD的杀菌效果呈现剂量反应模式，证实了它们作为治疗剂的潜力。这些肽表现出最小的细胞毒性和溶血作用，突出了它们的安全性。进一步的实验表明，天蚕素可以破坏细菌外膜，将脂质A作为靶点，脂质A是外膜中脂多糖（LPS）的关键成分，是它们的抗菌核心。天蚕素对LPS的亲和力及其抗菌作用突出了这些肽在治疗革兰氏阴性菌感染方面的潜力。这些见解凸显了天蚕素作为可行的“抗生素替代品”的前景，为其在对抗抗生素耐药性方面的广泛应用铺平了道路。
