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通过在小鼠体内反复进行心内注射来生成脑转移瘤细胞的实验方案。

Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice.

作者信息

Wang Li, Chen Xiaoxia, Dong Chengtao, Yin Shengqi, Liang Li, Zhou Aidong

机构信息

Department of Pathology, Nanfang Hospital, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Guangdong Province Key Laboratory of Molecular Tumor Pathology, Southern Medical University, Guangzhou 510515, China.

Department of Cell Biology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103531. doi: 10.1016/j.xpro.2024.103531. Epub 2024 Dec 20.

DOI:10.1016/j.xpro.2024.103531
PMID:39708324
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11726779/
Abstract

Here, we present a protocol for generating the lung cancer cell line, LLC1-BMT5, with highly brain metastatic tropism through multiple rounds of in vivo selection. We describe steps for establishing the brain metastases (BrMs) mouse model through intracardiac injection of cancer cells. We then detail procedures for obtaining brain metastatic subpopulations, in vivo selection of LLC1-BMT5 cells, and validating metastatic potential. This protocol will facilitate the study of the molecular mechanisms of BrMs and the development of anti-BrM treatment strategies. For complete details on the use and execution of this protocol, please refer to Wang et al..

摘要

在此，我们展示了一种通过多轮体内筛选生成具有高度脑转移倾向的肺癌细胞系LLC1-BMT5的方案。我们描述了通过心内注射癌细胞建立脑转移（BrMs）小鼠模型的步骤。然后，我们详细介绍了获得脑转移亚群、体内筛选LLC1-BMT5细胞以及验证转移潜能的程序。该方案将有助于研究脑转移的分子机制和开发抗脑转移治疗策略。有关该方案的使用和执行的完整详细信息，请参考Wang等人的研究。

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本文引用的文献

1
Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.靶向 HSP47-胶原轴通过逆转 M2 小胶质细胞极化和恢复抗肿瘤免疫来抑制脑转移。
Cell Rep Med. 2024 May 21;5(5):101533. doi: 10.1016/j.xcrm.2024.101533. Epub 2024 May 13.
2
Current approaches to the management of brain metastases.脑转移瘤的治疗方法。
Nat Rev Clin Oncol. 2020 May;17(5):279-299. doi: 10.1038/s41571-019-0320-3. Epub 2020 Feb 20.
3
A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis.
TAZ-AXL-ABL2 正向信号轴促进肺腺癌脑转移。
Cell Rep. 2019 Dec 10;29(11):3421-3434.e8. doi: 10.1016/j.celrep.2019.11.018.
4
The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges.上皮细胞-间充质转化中的 E-钙黏蛋白和 N-钙黏蛋白转换：信号转导、治疗意义和挑战。
Cells. 2019 Sep 20;8(10):1118. doi: 10.3390/cells8101118.
5
Brain metastases.脑转移瘤。
Nat Rev Dis Primers. 2019 Jan 17;5(1):5. doi: 10.1038/s41572-018-0055-y.
6
Serpins promote cancer cell survival and vascular co-option in brain metastasis.丝氨酸蛋白酶抑制剂促进脑转移中的癌细胞存活和血管选择。
Cell. 2014 Feb 27;156(5):1002-16. doi: 10.1016/j.cell.2014.01.040.