体外细胞培养模型中从吞噬耐甲氧西林金黄色葡萄球菌的肺泡巨噬细胞分离细胞外囊泡的方案。
Protocol for isolating extracellular vesicles from alveolar macrophages phagocytosing MRSA in vitro cell culture models.
作者信息
Bai Songjie, Hu Ruomeng, Wen Xuehuan, Zhang Wenting, Li Bingyu, Yang Jie, Yu Qing, Zeng Xianchang, Xiu Huiqing, Cai Zhijian, Zhang Gensheng
机构信息
Department of Critical Care Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Department of Cardiovascular Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, China.
Department of Critical Care Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103526. doi: 10.1016/j.xpro.2024.103526. Epub 2024 Dec 20.
Extracellular vesicles (EVs) play a crucial role in delivering bioactive cargo in infectious diseases. Here, we present a protocol for isolating EVs from alveolar macrophages (AMs) that phagocytose methicillin-resistant Staphylococcus aureus (MRSA) in vitro cell culture models. We describe steps for bacterial preparation; infection of AMs with MRSA; and isolation, purification, and characterization of EVs. This protocol provides a valuable perspective for studying EVs derived from pathogen-infected immune cells. For complete details on the use and execution of this protocol, please refer to Bai et al..
细胞外囊泡（EVs）在传染病中生物活性物质的传递过程中起着关键作用。在此，我们介绍一种从体外细胞培养模型中吞噬耐甲氧西林金黄色葡萄球菌（MRSA）的肺泡巨噬细胞（AMs）中分离EVs的方法。我们描述了细菌制备、AMs用MRSA感染以及EVs的分离、纯化和表征的步骤。该方法为研究源自病原体感染免疫细胞的EVs提供了有价值的视角。有关该方法的使用和实施的完整详细信息，请参考Bai等人的研究。
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