Yang Jing, Shen Liping, Zhao Yiqing, Zhou Xingyu, Liu Yongping
School of Chemistry and Chemical Engineering, North University of China, No.3 Xueyuan Road, Jiancaoping District, Taiyuan 030051, China; Shanxi Jingxi Biotechnology Co. Ltd, Taiyuan, Shanxi 030051, China.
School of Chemistry and Chemical Engineering, North University of China, No.3 Xueyuan Road, Jiancaoping District, Taiyuan 030051, China.
Int J Biol Macromol. 2025 Feb;291:138923. doi: 10.1016/j.ijbiomac.2024.138923. Epub 2024 Dec 19.
This study developed PQSp wound dressing hydrogels (S0-S6) using polyvinyl alcohol (PVA), quaternary chitosan (QCS), and sodium alginate (SA) as the matrix, with the addition of coconut mesocarp polyphenol (P-CTP, 0.1 %, 0.5 %, and 1.0 %) and β-glycerophosphate disodium (GP, 1.0 %) through a freeze-thaw method. Compared to hydrogels without P-CTP and GP (S0), the tensile strength of S1-S6 increased from 0.08 MPa to 0.45 MPa, elongation at break improved from 200 % to 320 %, and the swelling ratio decreased from 186 % to 82 % due to the effects of P-CTP and GP, while maintaining water content above 80 %, ensuring a moist environment for wound healing. Their thermal stability was also improved. SEM, FTIR, and XPS results confirmed enhanced crosslinking within the multi-network of the hydrogels, attributed to the increased hydrogen bonding from GP and P-CTP, independent of chemical crosslinking. However, antioxidant and antibacterial activities were dose-dependent only on P-CTP, with S3 and S6 showing the best effects. CAM and chicken embryo assays confirmed the hydrogels' non-toxicity and biocompatibility. These findings suggest that PQSp hydrogels, with their excellent mechanical properties, bioactivity, and safety, hold great potential for advanced wound dressing applications and provide a reference for expanding the application range of P-CTP.
本研究采用聚乙烯醇（PVA）、季铵化壳聚糖（QCS）和海藻酸钠（SA）作为基质，通过冻融法添加椰壳中果皮多酚（P-CTP，0.1%、0.5%和1.0%）和β-甘油磷酸二钠（GP，1.0%），制备了PQSp伤口敷料水凝胶（S0-S6）。与不含P-CTP和GP的水凝胶（S0）相比，由于P-CTP和GP的作用，S1-S6的拉伸强度从0.08MPa提高到0.45MPa，断裂伸长率从200%提高到320%，溶胀率从186%降低到82%，同时含水量保持在80%以上，为伤口愈合确保了湿润环境。它们的热稳定性也得到了提高。扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）结果证实了水凝胶多网络内交联增强，这归因于GP和P-CTP增加的氢键作用，与化学交联无关。然而，抗氧化和抗菌活性仅对P-CTP呈剂量依赖性，S3和S6表现出最佳效果。鸡胚绒毛尿囊膜（CAM）和鸡胚试验证实了水凝胶的无毒性和生物相容性。这些发现表明，PQSp水凝胶具有优异的力学性能、生物活性和安全性，在高级伤口敷料应用中具有巨大潜力，并为扩大P-CTP的应用范围提供了参考。
