Chen Xiuqin, Zheng Min, Lin Su, Huang Meiqing, Chen Shaoying, Chen Shilong
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, 350013, China.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou, Fujian, 350013, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian, 350013, China.
Mol Cell Probes. 2025 Feb;79:102004. doi: 10.1016/j.mcp.2024.102004. Epub 2024 Dec 21.
High-throughput genetic screening serves as an indispensable approach for deciphering gene functions and the intricate relationships between phenotypes and genotypes. The CRISPR/Cas9 system, with its ability to precisely edit genomes on a large scale, has revolutionized the field by enabling the construction of comprehensive genomic libraries. This technology has become a cornerstone for genome-wide screenings in disease research. This review offers a comprehensive examination of how CRISPR/Cas9-based genetic screening has been leveraged to uncover genes that play a role in disease mechanisms, focusing on areas such as cancer development and viral replication processes. The insights presented in this review hold promise for the development of novel therapeutic strategies and precision medicine approaches.
高通量基因筛选是解析基因功能以及表型与基因型之间复杂关系不可或缺的方法。CRISPR/Cas9系统能够大规模精确编辑基因组，通过构建全面的基因组文库彻底改变了该领域。这项技术已成为疾病研究中全基因组筛选的基石。本综述全面探讨了基于CRISPR/Cas9的基因筛选如何用于揭示在疾病机制中起作用的基因，重点关注癌症发展和病毒复制过程等领域。本综述中提出的见解为开发新型治疗策略和精准医学方法带来了希望。
