Kim S, Jacobs R E, White S H
Biochim Biophys Acta. 1985 Feb 14;812(3):793-801. doi: 10.1016/0005-2736(85)90274-3.
A novel method of preparing multilamellar vesicles is described. The process involves dispersing in aqueous solutions small spherules of volatile hydrophobic solvents in which amphipathic lipids are dissolved. The lipids form vesicles when the solvents are evaporated in the proper manner. The resulting vesicles have been characterized morphologically with microscopy and electron microscopy. The method yields multilamellar vesicles with a defined size distribution which can be adjusted by varying the duration of mechanical agitation of the spherules and by varying the concentration of amphipathic lipids in the solvents. This is the first fundamentally new method of multilamellar vesicle preparation since Bangham's report in 1965 (Bangham, A.D., Standish, M.M. and Watkins, J.C. (1965) J. Mol. Biol. 13, 238-252).
描述了一种制备多层囊泡的新方法。该过程包括将挥发性疏水溶剂的小球体分散在水溶液中，两亲性脂质溶解在这些小球体中。当溶剂以适当方式蒸发时，脂质形成囊泡。所得囊泡已通过显微镜和电子显微镜进行了形态学表征。该方法产生具有确定尺寸分布的多层囊泡，可通过改变小球体的机械搅拌持续时间以及改变溶剂中两亲性脂质的浓度来进行调节。这是自1965年班汉姆报告（班汉姆，A.D.，斯坦迪什，M.M.和沃特金斯，J.C.（1965年）《分子生物学杂志》13卷，238 - 252页）以来第一种全新的多层囊泡制备方法。
