Olson F, Hunt C A, Szoka F C, Vail W J, Papahadjopoulos D
Biochim Biophys Acta. 1979 Oct 19;557(1):9-23. doi: 10.1016/0005-2736(79)90085-3.
Liposomes of defined size and homogeneity have been prepared by sequential extrusion of the usual multilamellar vesicles through polycarbonate membranes. The process is easy, reproducible, produces no detectable degradation of the phospholipids, and can double the encapsulation efficiency of the liposome preparation. Multilamellar vesicles extruded by this technique are shown by both negative stain and freeze-fracture electron microscopy to have mean diameters approaching the pore diameter of the polycarbonate membrane through which they were extruded. When sequentially extruded down through a 0.2 micron membrane, the resulting vesicles exhibit a very homogeneous size distribution with a mean diameter of 0.27 micron while maintaining an acceptable level of encapsulation of the aqueous phase.
通过将普通多层囊泡依次挤压通过聚碳酸酯膜，制备出了尺寸确定且均一的脂质体。该过程简便、可重复，不会导致磷脂出现可检测到的降解，并且能使脂质体制剂的包封效率提高一倍。通过这种技术挤压得到的多层囊泡，经负染和冷冻断裂电子显微镜观察显示，其平均直径接近挤压时所通过的聚碳酸酯膜的孔径。当依次向下通过0.2微米的膜进行挤压时，所得囊泡呈现出非常均一的尺寸分布，平均直径为0.27微米，同时保持水相的包封率处于可接受水平。
